Cell-free protein expression from DNA-based hydrogel (P-Gel) droplets for scale-up production

Roanna C.H. Ruiz, Pichamon Kiatwuthinon, Jason S. Kahn, Young Hoon Roh, Dan Luo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This paper presents a promising potential scale-up method for cell-free protein production that integrates microfluidic devices and DNA-based hydrogels (P-gels). The microfluidic devices were fabricated using established photolithography techniques. The P-gels were formed via enzymatic ligation of branched DNA nanostructures with genes. More specifically, the microfluidic devices contained droplet-forming junctions through which numerous micron-sized P-gel droplets were produced. The advantages of our microfluidic-based approach include highly controlled fluid manipulation, rapid droplet generation, high reproducibility, and low sample consumption. P-gel droplets successfully expressed green fluorescent protein (GFP) in high yield, and the microfluidic devices achieved a variety of P-gel droplet sizes while maintaining uniform morphology and a high generation rate. We envision that our system could provide a cell-free platform for both high-throughput and scale-up production of multiple proteins.

Original languageEnglish
Pages (from-to)372-377
Number of pages6
JournalIndustrial Biotechnology
Volume8
Issue number6
DOIs
Publication statusPublished - 2012 Dec 1

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All Science Journal Classification (ASJC) codes

  • Biotechnology

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