Cell immobilization on polymer by air atmospheric pressure plasma jet treatment

Jung Hwan Lee, Jae Sung Kwon, Ji Yeon Om, Yong Hee Kim, Eun Ha Choi, Kwang Mahn Kim, Kyoung Nam Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The study of cell immobilization on delicate polymer by an air atmospheric pressure plasma jet (AAPPJ) is required for its medical application. The aim of this study was to evaluate whether AAPPJ treatment induce cell immobilization effect on delicate polymers without significant change of surface roughness by AAPPJ treatment. After surface roughness, dynamic contact angle, and chemical characteristics were investigated, the immobilization effect was evaluated with the mouse fibroblast L929 cell line. Surface roughness change was not observed (P > 0.05) in either delicate dental wax or polystyrene plate (PSP) as advancing and receding contact angles significantly decreased (P < 0.05), thanks to decreased hydrocarbon and formation of oxygen-related functional groups in treated PSP. Adherent L929 cells with elongated morphology were found in treated PSP along with the formation of immobilization markers vinculin and actin cytoskeleton. Increased PTK2 gene expression upregulated these markers on treated PSP.

Original languageEnglish
Article number086202
JournalJapanese Journal of Applied Physics
Volume53
Issue number8
DOIs
Publication statusPublished - 2014 Aug

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

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