Cell migration according to shape of graphene oxide micropatterns

Sung Eun Kim, Min Sung Kim, Yong Cheol Shin, Seong Un Eom, Jong Ho Lee, Dong Myeong Shin, Suck Won Hong, Bongju Kim, Jong Chul Park, Bo Sung Shin, Dohyung Lim, Dong Wook Han

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds.

Original languageEnglish
Article number186
JournalMicromachines
Volume7
Issue number10
DOIs
Publication statusPublished - 2016 Oct 14

Fingerprint

Graphene
Oxides
Photolithography
Tissue engineering
Substrates
Hydrophilicity
Scaffolds (biology)
Drug delivery
Raman spectroscopy
Atomic force microscopy
Proteins
Scanning electron microscopy

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Kim, S. E., Kim, M. S., Shin, Y. C., Eom, S. U., Lee, J. H., Shin, D. M., ... Han, D. W. (2016). Cell migration according to shape of graphene oxide micropatterns. Micromachines, 7(10), [186]. https://doi.org/10.3390/mi7100186
Kim, Sung Eun ; Kim, Min Sung ; Shin, Yong Cheol ; Eom, Seong Un ; Lee, Jong Ho ; Shin, Dong Myeong ; Hong, Suck Won ; Kim, Bongju ; Park, Jong Chul ; Shin, Bo Sung ; Lim, Dohyung ; Han, Dong Wook. / Cell migration according to shape of graphene oxide micropatterns. In: Micromachines. 2016 ; Vol. 7, No. 10.
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Kim, SE, Kim, MS, Shin, YC, Eom, SU, Lee, JH, Shin, DM, Hong, SW, Kim, B, Park, JC, Shin, BS, Lim, D & Han, DW 2016, 'Cell migration according to shape of graphene oxide micropatterns', Micromachines, vol. 7, no. 10, 186. https://doi.org/10.3390/mi7100186

Cell migration according to shape of graphene oxide micropatterns. / Kim, Sung Eun; Kim, Min Sung; Shin, Yong Cheol; Eom, Seong Un; Lee, Jong Ho; Shin, Dong Myeong; Hong, Suck Won; Kim, Bongju; Park, Jong Chul; Shin, Bo Sung; Lim, Dohyung; Han, Dong Wook.

In: Micromachines, Vol. 7, No. 10, 186, 14.10.2016.

Research output: Contribution to journalArticle

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T1 - Cell migration according to shape of graphene oxide micropatterns

AU - Kim, Sung Eun

AU - Kim, Min Sung

AU - Shin, Yong Cheol

AU - Eom, Seong Un

AU - Lee, Jong Ho

AU - Shin, Dong Myeong

AU - Hong, Suck Won

AU - Kim, Bongju

AU - Park, Jong Chul

AU - Shin, Bo Sung

AU - Lim, Dohyung

AU - Han, Dong Wook

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AB - Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds.

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Kim SE, Kim MS, Shin YC, Eom SU, Lee JH, Shin DM et al. Cell migration according to shape of graphene oxide micropatterns. Micromachines. 2016 Oct 14;7(10). 186. https://doi.org/10.3390/mi7100186