Cell-niche-on-chip: Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes

Yu Chih Chen, Yu Heng Cheng, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a novel design of single cell-cell interaction platform for studying tumor-stromal interaction. In cell-cell interaction assays, monitoring single cell behavior is critical to study highly heterogeneous population (e.g., cancer). For example, metastatic cancer cells are typically transported as a single circulating tumor cell; therefore, tumorigenesis should be induced from a single cell because it might be quite different from co-culturing many cells. In this work, we report a microfluidic chip that allows for isolating a pair of cells by using immiscible liquid isolation. We present the method of using a semi-permeable membrane to retain secreted proteins for interaction, while maintaining the exchange of nutrition for long-term cell-cell interaction studies. We verified proliferation enhancement of tumor cells when co-cultured with stromal cells, demonstrating the feasibility of our device as a generic platform of cell-cell interaction studies.

Original languageEnglish
Title of host publicationMEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages943-946
Number of pages4
ISBN (Print)9781479935086
DOIs
Publication statusPublished - 2014
Event27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States
Duration: 2014 Jan 262014 Jan 30

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
CountryUnited States
CitySan Francisco, CA
Period14/1/2614/1/30

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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    Chen, Y. C., Cheng, Y. H., & Yoon, E. (2014). Cell-niche-on-chip: Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 943-946). [6765798] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765798