Cell-niche-on-chip: Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes

Yu Chih Chen, Yu Heng Cheng, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a novel design of single cell-cell interaction platform for studying tumor-stromal interaction. In cell-cell interaction assays, monitoring single cell behavior is critical to study highly heterogeneous population (e.g., cancer). For example, metastatic cancer cells are typically transported as a single circulating tumor cell; therefore, tumorigenesis should be induced from a single cell because it might be quite different from co-culturing many cells. In this work, we report a microfluidic chip that allows for isolating a pair of cells by using immiscible liquid isolation. We present the method of using a semi-permeable membrane to retain secreted proteins for interaction, while maintaining the exchange of nutrition for long-term cell-cell interaction studies. We verified proliferation enhancement of tumor cells when co-cultured with stromal cells, demonstrating the feasibility of our device as a generic platform of cell-cell interaction studies.

Original languageEnglish
Title of host publicationMEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages943-946
Number of pages4
ISBN (Print)9781479935086
DOIs
Publication statusPublished - 2014 Jan 1
Event27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States
Duration: 2014 Jan 262014 Jan 30

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
CountryUnited States
CitySan Francisco, CA
Period14/1/2614/1/30

Fingerprint

Tumors
isolation
platforms
chips
Cells
membranes
Membranes
Liquids
liquids
cells
Nutrition
Microfluidics
Assays
Proteins
tumors
Monitoring
interactions
cancer
nutrition
proteins

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Chen, Y. C., Cheng, Y. H., & Yoon, E. (2014). Cell-niche-on-chip: Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 943-946). [6765798] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765798
Chen, Yu Chih ; Cheng, Yu Heng ; Yoon, Euisik. / Cell-niche-on-chip : Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes. MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 943-946 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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Chen, YC, Cheng, YH & Yoon, E 2014, Cell-niche-on-chip: Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765798, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 943-946, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 14/1/26. https://doi.org/10.1109/MEMSYS.2014.6765798

Cell-niche-on-chip : Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes. / Chen, Yu Chih; Cheng, Yu Heng; Yoon, Euisik.

MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. p. 943-946 6765798 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen YC, Cheng YH, Yoon E. Cell-niche-on-chip: Paired single cell co-culture platforms using immiscible liquid isolation and semi-permeable membranes. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2014. p. 943-946. 6765798. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2014.6765798