Cell-penetrating DNA-binding protein as a safe and efficient naked DNA delivery carrier in vitro and in vivo

Eun Sung Kim, Seung Woo Yang, Dong Ki Hong, Woo Taek Kim, Ho Guen Kim, Sang Kyou Lee

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Non-viral gene delivery is a safe and suitable alternative to viral vector-mediated delivery to overcome the immunogenicity and tumorigenesis associated with viral vectors. Using the novel, human-origin Hph-1 protein transduction domain that can facilitate the transduction of protein into cells, we developed a new strategy to deliver naked DNA in vitro and in vivo. The new DNA delivery system contains Hph-1-GAL4 DNA-binding domain (DBD) fusion protein and enhanced green fluorescent protein (EGFP) reporter plasmid that includes the five repeats of GAL4 upstream activating sequence (UAS). Hph-1-GAL4-DBD protein formed complex with plasmid DNA through the specific interaction between GAL4-DBD and UAS, and delivered into the cells via the Hph-1-PTD. The pEGFP DNA was successfully delivered by the Hph-1-GAL4 system, and the EGFP was effectively expressed in mammalian cells such as HeLa and Jurkat, as well as in Bright Yellow-2 (BY-2) plant cells. When 10 μg of pEGFP DNA was intranasally administered to mice using Hph-1-GAL4 protein, a high level of EGFP expression was detected throughout the lung tissue for 7 days. These results suggest that an Hph-1-PTD-mediated DNA delivery strategy may be an useful non-viral DNA delivery system for gene therapy and DNA vaccines.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume392
Issue number1
DOIs
Publication statusPublished - 2010 Jan 29

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea Grant Funded by the Korean Government ( 20090065531 and 20090081587 ) and in part by Brain Korea 21 Program ( 2009-1-0012 ).

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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