Cell surface antigen display for neuronal differentiation-specific tracking

Sang Chul Kim, Eun Hye Lee, Ji Hea Yu, Sang Mi Kim, Bae Geun Nam, Hee Yong Chung, Yeon Soo Kim, Sung Rae Cho, Chang Hwan Park

Research output: Contribution to journalArticle

Abstract

Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5’ UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalBiomolecules and Therapeutics
Volume27
Issue number1
DOIs
Publication statusPublished - 2019 Jan

Fingerprint

Neural Stem Cells
Surface Antigens
Stem cells
Display devices
Neurons
Synapsins
5' Untranslated Regions
Brain Diseases
Cell- and Tissue-Based Therapy
Cell membranes
Stem Cells
Cell Membrane
Technology
Brain
Cells
Tissue
Antigens
Monitoring
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

Cite this

Kim, S. C., Lee, E. H., Yu, J. H., Kim, S. M., Nam, B. G., Chung, H. Y., ... Park, C. H. (2019). Cell surface antigen display for neuronal differentiation-specific tracking. Biomolecules and Therapeutics, 27(1), 78-84. https://doi.org/10.4062/biomolther.2018.110
Kim, Sang Chul ; Lee, Eun Hye ; Yu, Ji Hea ; Kim, Sang Mi ; Nam, Bae Geun ; Chung, Hee Yong ; Kim, Yeon Soo ; Cho, Sung Rae ; Park, Chang Hwan. / Cell surface antigen display for neuronal differentiation-specific tracking. In: Biomolecules and Therapeutics. 2019 ; Vol. 27, No. 1. pp. 78-84.
@article{6c57e0a2c8ba401d93765804ad7a1af2,
title = "Cell surface antigen display for neuronal differentiation-specific tracking",
abstract = "Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5’ UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.",
author = "Kim, {Sang Chul} and Lee, {Eun Hye} and Yu, {Ji Hea} and Kim, {Sang Mi} and Nam, {Bae Geun} and Chung, {Hee Yong} and Kim, {Yeon Soo} and Cho, {Sung Rae} and Park, {Chang Hwan}",
year = "2019",
month = "1",
doi = "10.4062/biomolther.2018.110",
language = "English",
volume = "27",
pages = "78--84",
journal = "Biomolecules and Therapeutics",
issn = "1976-9148",
publisher = "Korean Society of Applied Pharmacology",
number = "1",

}

Kim, SC, Lee, EH, Yu, JH, Kim, SM, Nam, BG, Chung, HY, Kim, YS, Cho, SR & Park, CH 2019, 'Cell surface antigen display for neuronal differentiation-specific tracking', Biomolecules and Therapeutics, vol. 27, no. 1, pp. 78-84. https://doi.org/10.4062/biomolther.2018.110

Cell surface antigen display for neuronal differentiation-specific tracking. / Kim, Sang Chul; Lee, Eun Hye; Yu, Ji Hea; Kim, Sang Mi; Nam, Bae Geun; Chung, Hee Yong; Kim, Yeon Soo; Cho, Sung Rae; Park, Chang Hwan.

In: Biomolecules and Therapeutics, Vol. 27, No. 1, 01.2019, p. 78-84.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cell surface antigen display for neuronal differentiation-specific tracking

AU - Kim, Sang Chul

AU - Lee, Eun Hye

AU - Yu, Ji Hea

AU - Kim, Sang Mi

AU - Nam, Bae Geun

AU - Chung, Hee Yong

AU - Kim, Yeon Soo

AU - Cho, Sung Rae

AU - Park, Chang Hwan

PY - 2019/1

Y1 - 2019/1

N2 - Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5’ UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

AB - Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5’ UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

UR - http://www.scopus.com/inward/record.url?scp=85067025926&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067025926&partnerID=8YFLogxK

U2 - 10.4062/biomolther.2018.110

DO - 10.4062/biomolther.2018.110

M3 - Article

AN - SCOPUS:85067025926

VL - 27

SP - 78

EP - 84

JO - Biomolecules and Therapeutics

JF - Biomolecules and Therapeutics

SN - 1976-9148

IS - 1

ER -