Cell surface antigen display for neuronal differentiation-specific tracking

Sang Chul Kim, Eun Hye Lee, Ji Hea Yu, Sang Mi Kim, Bae Geun Nam, Hee Yong Chung, Yeon Soo Kim, Sung Rae Cho, Chang Hwan Park

Research output: Contribution to journalArticle

Abstract

Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5’ UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalBiomolecules and Therapeutics
Volume27
Issue number1
DOIs
Publication statusPublished - 2019 Jan

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

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  • Cite this

    Kim, S. C., Lee, E. H., Yu, J. H., Kim, S. M., Nam, B. G., Chung, H. Y., Kim, Y. S., Cho, S. R., & Park, C. H. (2019). Cell surface antigen display for neuronal differentiation-specific tracking. Biomolecules and Therapeutics, 27(1), 78-84. https://doi.org/10.4062/biomolther.2018.110