Cell Transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer

Wing Fu Lai, Han Sung Jung

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

Original languageEnglish
Article numbere100258
JournalPloS one
Volume9
Issue number6
DOIs
Publication statusPublished - 2014 Jun 23

Fingerprint

Propane
Polyetherimides
propane
cyclodextrins
Cyclodextrins
transfection
Transfection
polymers
Polymers
cells
gene transfer
Gene transfer
Gene therapy
gene therapy
buffering capacity
DNA
Hep G2 Cells
Cytotoxicity
crosslinking
Genetic Therapy

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{c5abbef868ca4a1db50edc81447abca5,
title = "Cell Transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer",
abstract = "Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.",
author = "Lai, {Wing Fu} and Jung, {Han Sung}",
year = "2014",
month = "6",
day = "23",
doi = "10.1371/journal.pone.0100258",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

Cell Transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer. / Lai, Wing Fu; Jung, Han Sung.

In: PloS one, Vol. 9, No. 6, e100258, 23.06.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cell Transfection with a β-cyclodextrin-PEI-propane-1,2,3-triol nanopolymer

AU - Lai, Wing Fu

AU - Jung, Han Sung

PY - 2014/6/23

Y1 - 2014/6/23

N2 - Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

AB - Successful gene therapy necessitates safe and efficient gene transfer. This article describes the use of a cationic polymer, which was synthesized by cross-linking low molecular weight branched poly(ethylenimine) (PEI) with both β-cyclodextrin and propane-1,2,3-triol, for efficient and safe non-viral gene delivery. Experimentation demonstrated that the polymer had a pH buffering capacity and DNA condensing ability comparable to those of PEI 25 kDa. In B16-F0 cells, the polymer increased the transfection efficiency of naked DNA by 700-fold and yielded better transfection efficiencies than Fugene HD (threefold higher) and PEI 25 kDa (fivefold higher). The high transfection efficiency of the polymer was not affected by the presence of serum during transfection. In addition to B16-F0 cells, the polymer enabled efficient transfection of HepG2 and U87 cells with low cytotoxicity. Our results indicated that our polymer is a safe and efficient transfection reagent that warrants further development for in vitro, in vivo and clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=84903525919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903525919&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0100258

DO - 10.1371/journal.pone.0100258

M3 - Article

C2 - 24956480

AN - SCOPUS:84903525919

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e100258

ER -