Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death

Y. Cho, K. H. Huh, Y. J. Park, J. H. Do, D. J. Joo, M. S. Kim, Y. S. Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-α and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-α during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-α and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-α to be directly correlated. Rac1 binding to RhoGDI-α decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-α/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.

Original languageEnglish
Pages (from-to)1083-1085
Number of pages3
JournalTransplantation Proceedings
Issue number4
Publication statusPublished - 2012 May

Bibliographical note

Funding Information:
This work was supported by a faculty research grant ( #6-2010-0035 ) at Yonsei University College of Medicine . Yuri Cho and Ji Hye Do are research associates supported by BK21 for Medical Science ( 7-2006-0253 ) at Yonsei University College of Medicine.

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation


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