Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death

Y. Cho, K. H. Huh, Y. J. Park, J. H. Do, D. J. Joo, M. S. Kim, Y. S. Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-α and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-α during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-α and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-α to be directly correlated. Rac1 binding to RhoGDI-α decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-α/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.

Original languageEnglish
Pages (from-to)1083-1085
Number of pages3
JournalTransplantation Proceedings
Volume44
Issue number4
DOIs
Publication statusPublished - 2012 May 1

Fingerprint

Mycophenolic Acid
Insulin-Secreting Cells
Cell Death
Apoptosis
Immunoprecipitation
Pancreas Transplantation
Islets of Langerhans Transplantation
MAP Kinase Signaling System
Immunosuppressive Agents
Islets of Langerhans
Therapeutics
Down-Regulation
Cell Line

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Cho, Y. ; Huh, K. H. ; Park, Y. J. ; Do, J. H. ; Joo, D. J. ; Kim, M. S. ; Kim, Y. S. / Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death. In: Transplantation Proceedings. 2012 ; Vol. 44, No. 4. pp. 1083-1085.
@article{13607f757f1044f3bc353511df764854,
title = "Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death",
abstract = "Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-α and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-α during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-α and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-α to be directly correlated. Rac1 binding to RhoGDI-α decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-α/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.",
author = "Y. Cho and Huh, {K. H.} and Park, {Y. J.} and Do, {J. H.} and Joo, {D. J.} and Kim, {M. S.} and Kim, {Y. S.}",
year = "2012",
month = "5",
day = "1",
doi = "10.1016/j.transproceed.2012.03.021",
language = "English",
volume = "44",
pages = "1083--1085",
journal = "Transplantation Proceedings",
issn = "0041-1345",
publisher = "Elsevier USA",
number = "4",

}

Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death. / Cho, Y.; Huh, K. H.; Park, Y. J.; Do, J. H.; Joo, D. J.; Kim, M. S.; Kim, Y. S.

In: Transplantation Proceedings, Vol. 44, No. 4, 01.05.2012, p. 1083-1085.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cellular function of RhoGDI-α mediates the cycling of Rac1 and the regulation of pancreatic beta cell death

AU - Cho, Y.

AU - Huh, K. H.

AU - Park, Y. J.

AU - Do, J. H.

AU - Joo, D. J.

AU - Kim, M. S.

AU - Kim, Y. S.

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-α and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-α during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-α and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-α to be directly correlated. Rac1 binding to RhoGDI-α decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-α/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.

AB - Mycophenolic acid (MPA) is an immunosuppressive agent that is widely used in clinical therapy, including pancreas and islet transplantation. Previously, we showed that MPA induces significant apoptosis of insulin-secreting cells by downregulating RhoGDI-α and increasing JNK expression. In this study, we investigated Rac1 directly associated with RhoGDI-α during MPA-induced apoptosis in INS-1E cells (an insulin-secreting cell line). Cells were treated with MPA for 24 and 36 hours. Immunoprecipitation was used to examine physical interactions between RhoGDI-α and Rac1. Activation and immunoprecipitation assays showed expressions of Rac1 and RhoGDI-α to be directly correlated. Rac1 binding to RhoGDI-α decreased after MPA treatment, and Rac1 was induced and subsequently activated by MPA. We concluded that this novel RhoGDI-α/Rac1/JNK pathway induced apoptosis of transplanted islet cells after MPA treatment.

UR - http://www.scopus.com/inward/record.url?scp=84860731398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860731398&partnerID=8YFLogxK

U2 - 10.1016/j.transproceed.2012.03.021

DO - 10.1016/j.transproceed.2012.03.021

M3 - Article

C2 - 22564631

AN - SCOPUS:84860731398

VL - 44

SP - 1083

EP - 1085

JO - Transplantation Proceedings

JF - Transplantation Proceedings

SN - 0041-1345

IS - 4

ER -