Cellular stress-related protein expression in Helicobacter pylori-infected gastric epithelial AGS cells

Joo Weon Lim, Hyeyoung Kim, Jung Mogg Kim, Joo Sung Kim, Hyun Chae Jung, Kyung Hwan Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration, and gastric carcinoma. Moreover, H. pylori may induce disease-specific protein expression in gastric epithelial cells. The present study was aimed at determining differentially expressed proteins in H. pylori-infected gastric epithelial AGS cells. AGS cells were treated with H. pylori at a bacterium/cell ratio of 300:1 for 12 h. Altered protein patterns as separated by two-dimensional electrophoresis using pH gradients of 4-7 were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Four differentially expressed proteins, whose expression levels were increased by more than two-fold in H. pylori-infected cells, were analyzed. These proteins (14-3-3 protein α/β, cullin homolog 3, α-enolase, ezrin) are known to be related to cell proliferation, cell adhesion, and carcinogenesis, and may be mediated by cellular stress, such as reactive oxygen species. In conclusion, the identification of these differentially expressed proteins provide valuable information for the understanding of the pathophysiologic mechanisms of H. pylori-induced gastric diseases, and may be useful as prognostic indices of H. pylori-related gastric disorders.

Original languageEnglish
Pages (from-to)1624-1634
Number of pages11
JournalInternational Journal of Biochemistry and Cell Biology
Volume36
Issue number8
DOIs
Publication statusPublished - 2004 Aug 1

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Heat-Shock Proteins
Helicobacter pylori
Stomach
Epithelial Cells
14-3-3 Proteins
Proteins
Cullin Proteins
Phosphopyruvate Hydratase
Cell adhesion
Cell proliferation
Electrophoresis
Stomach Diseases
Ionization
Mass spectrometry
Proton-Motive Force
Reactive Oxygen Species
Desorption
Bacteria
Helicobacter Infections
Cell Adhesion

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology

Cite this

Lim, Joo Weon ; Kim, Hyeyoung ; Kim, Jung Mogg ; Kim, Joo Sung ; Jung, Hyun Chae ; Kim, Kyung Hwan. / Cellular stress-related protein expression in Helicobacter pylori-infected gastric epithelial AGS cells. In: International Journal of Biochemistry and Cell Biology. 2004 ; Vol. 36, No. 8. pp. 1624-1634.
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Cellular stress-related protein expression in Helicobacter pylori-infected gastric epithelial AGS cells. / Lim, Joo Weon; Kim, Hyeyoung; Kim, Jung Mogg; Kim, Joo Sung; Jung, Hyun Chae; Kim, Kyung Hwan.

In: International Journal of Biochemistry and Cell Biology, Vol. 36, No. 8, 01.08.2004, p. 1624-1634.

Research output: Contribution to journalArticle

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