Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia

Choong Hyun Lee, Joon Ha Park, Jeong Hwi Cho, Ji Hyeon Ahn, Bing Chun Yan, Jae Chul Lee, Myoung Cheol Shin, Seung Hwan Cheon, Young Shin Cho, Jun Hwi Cho, Young-Guen Kwon, Dong Keon Lee, Young Myeong Kim, Moo Ho Won

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Redd1 (known as RTP801/Dig2/DDIT4) is a stress-induced protein, and it is known to be regulated in response to some stresses including hypoxia and oxidative stress. In the present study, we investigated the time-dependent changes in Redd1 immunoreactivity and its protein levels in the gerbil hippocampus proper (CA1-3 regions) after 5 min of transient global cerebral ischemia using immunohistochemistry and Western blot analysis. Redd1 immunoreactivity was apparently changed in the pyramidal neurons of the ischemic CA1 region, not in the pyramidal neurons of the ischemic CA2/3 region. Redd1 immunoreactivity in the CA1 pyramidal neurons was significantly increased at 6 h post-ischemia, decreased until 1 day post-ischemia, increased again at 2 days post-ischemia and weakly observed at 5 days post-ischemia. Especially, at 5 days after ischemic damage, Redd1 immunoreactivity was newly expressed in astrocytes and GABAergic interneurons in the CA1 region. Redd1 protein levels in the ischemic CA1 region were changed like the pattern of the Redd1 immunoreactivity. These results indicate that Redd1 immunoreactivity and protein levels are increased in the ischemic CA1 region at an early time after ischemic damage and that the increased Redd1 expression may be closely related to the delayed neuronal death of the CA1 pyramidal neurons following 5 min of transient global cerebral ischemia.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalJournal of the Neurological Sciences
Volume344
Issue number1-2
DOIs
Publication statusPublished - 2014 Sep 15

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Gerbillinae
Pyramidal Cells
Transient Ischemic Attack
Neuroglia
Hippocampus
Ischemia
Neurons
Proteins
Interneurons
Heat-Shock Proteins
Astrocytes
Oxidative Stress
Western Blotting
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Lee, Choong Hyun ; Park, Joon Ha ; Cho, Jeong Hwi ; Ahn, Ji Hyeon ; Yan, Bing Chun ; Lee, Jae Chul ; Shin, Myoung Cheol ; Cheon, Seung Hwan ; Cho, Young Shin ; Cho, Jun Hwi ; Kwon, Young-Guen ; Lee, Dong Keon ; Kim, Young Myeong ; Won, Moo Ho. / Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia. In: Journal of the Neurological Sciences. 2014 ; Vol. 344, No. 1-2. pp. 43-50.
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title = "Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia",
abstract = "Redd1 (known as RTP801/Dig2/DDIT4) is a stress-induced protein, and it is known to be regulated in response to some stresses including hypoxia and oxidative stress. In the present study, we investigated the time-dependent changes in Redd1 immunoreactivity and its protein levels in the gerbil hippocampus proper (CA1-3 regions) after 5 min of transient global cerebral ischemia using immunohistochemistry and Western blot analysis. Redd1 immunoreactivity was apparently changed in the pyramidal neurons of the ischemic CA1 region, not in the pyramidal neurons of the ischemic CA2/3 region. Redd1 immunoreactivity in the CA1 pyramidal neurons was significantly increased at 6 h post-ischemia, decreased until 1 day post-ischemia, increased again at 2 days post-ischemia and weakly observed at 5 days post-ischemia. Especially, at 5 days after ischemic damage, Redd1 immunoreactivity was newly expressed in astrocytes and GABAergic interneurons in the CA1 region. Redd1 protein levels in the ischemic CA1 region were changed like the pattern of the Redd1 immunoreactivity. These results indicate that Redd1 immunoreactivity and protein levels are increased in the ischemic CA1 region at an early time after ischemic damage and that the increased Redd1 expression may be closely related to the delayed neuronal death of the CA1 pyramidal neurons following 5 min of transient global cerebral ischemia.",
author = "Lee, {Choong Hyun} and Park, {Joon Ha} and Cho, {Jeong Hwi} and Ahn, {Ji Hyeon} and Yan, {Bing Chun} and Lee, {Jae Chul} and Shin, {Myoung Cheol} and Cheon, {Seung Hwan} and Cho, {Young Shin} and Cho, {Jun Hwi} and Young-Guen Kwon and Lee, {Dong Keon} and Kim, {Young Myeong} and Won, {Moo Ho}",
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Lee, CH, Park, JH, Cho, JH, Ahn, JH, Yan, BC, Lee, JC, Shin, MC, Cheon, SH, Cho, YS, Cho, JH, Kwon, Y-G, Lee, DK, Kim, YM & Won, MH 2014, 'Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia', Journal of the Neurological Sciences, vol. 344, no. 1-2, pp. 43-50. https://doi.org/10.1016/j.jns.2014.06.016

Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia. / Lee, Choong Hyun; Park, Joon Ha; Cho, Jeong Hwi; Ahn, Ji Hyeon; Yan, Bing Chun; Lee, Jae Chul; Shin, Myoung Cheol; Cheon, Seung Hwan; Cho, Young Shin; Cho, Jun Hwi; Kwon, Young-Guen; Lee, Dong Keon; Kim, Young Myeong; Won, Moo Ho.

In: Journal of the Neurological Sciences, Vol. 344, No. 1-2, 15.09.2014, p. 43-50.

Research output: Contribution to journalArticle

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T1 - Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia

AU - Lee, Choong Hyun

AU - Park, Joon Ha

AU - Cho, Jeong Hwi

AU - Ahn, Ji Hyeon

AU - Yan, Bing Chun

AU - Lee, Jae Chul

AU - Shin, Myoung Cheol

AU - Cheon, Seung Hwan

AU - Cho, Young Shin

AU - Cho, Jun Hwi

AU - Kwon, Young-Guen

AU - Lee, Dong Keon

AU - Kim, Young Myeong

AU - Won, Moo Ho

PY - 2014/9/15

Y1 - 2014/9/15

N2 - Redd1 (known as RTP801/Dig2/DDIT4) is a stress-induced protein, and it is known to be regulated in response to some stresses including hypoxia and oxidative stress. In the present study, we investigated the time-dependent changes in Redd1 immunoreactivity and its protein levels in the gerbil hippocampus proper (CA1-3 regions) after 5 min of transient global cerebral ischemia using immunohistochemistry and Western blot analysis. Redd1 immunoreactivity was apparently changed in the pyramidal neurons of the ischemic CA1 region, not in the pyramidal neurons of the ischemic CA2/3 region. Redd1 immunoreactivity in the CA1 pyramidal neurons was significantly increased at 6 h post-ischemia, decreased until 1 day post-ischemia, increased again at 2 days post-ischemia and weakly observed at 5 days post-ischemia. Especially, at 5 days after ischemic damage, Redd1 immunoreactivity was newly expressed in astrocytes and GABAergic interneurons in the CA1 region. Redd1 protein levels in the ischemic CA1 region were changed like the pattern of the Redd1 immunoreactivity. These results indicate that Redd1 immunoreactivity and protein levels are increased in the ischemic CA1 region at an early time after ischemic damage and that the increased Redd1 expression may be closely related to the delayed neuronal death of the CA1 pyramidal neurons following 5 min of transient global cerebral ischemia.

AB - Redd1 (known as RTP801/Dig2/DDIT4) is a stress-induced protein, and it is known to be regulated in response to some stresses including hypoxia and oxidative stress. In the present study, we investigated the time-dependent changes in Redd1 immunoreactivity and its protein levels in the gerbil hippocampus proper (CA1-3 regions) after 5 min of transient global cerebral ischemia using immunohistochemistry and Western blot analysis. Redd1 immunoreactivity was apparently changed in the pyramidal neurons of the ischemic CA1 region, not in the pyramidal neurons of the ischemic CA2/3 region. Redd1 immunoreactivity in the CA1 pyramidal neurons was significantly increased at 6 h post-ischemia, decreased until 1 day post-ischemia, increased again at 2 days post-ischemia and weakly observed at 5 days post-ischemia. Especially, at 5 days after ischemic damage, Redd1 immunoreactivity was newly expressed in astrocytes and GABAergic interneurons in the CA1 region. Redd1 protein levels in the ischemic CA1 region were changed like the pattern of the Redd1 immunoreactivity. These results indicate that Redd1 immunoreactivity and protein levels are increased in the ischemic CA1 region at an early time after ischemic damage and that the increased Redd1 expression may be closely related to the delayed neuronal death of the CA1 pyramidal neurons following 5 min of transient global cerebral ischemia.

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