Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy

Ah Young Leem, Joo Han Song, Eun Hye Lee, Hyejon Lee, Bora Sim, Song Yee Kim, Kyung Soo Chung, Eun Young Kim, Ji Ye Jung, Moo Suk Park, Young Sam Kim, Joon Chang, youngae kang

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Abstract

Multiple cytokines and inflammatory markers control TB infection. We aimed to investigate the changes in multiple cytokines and inflammatory markers in active TB patients following anti-TB drug therapy. Twenty-nine patients with active TB were recruited prospectively between December 2010 and July 2017. Blood samples were collected before (T 0 ), after 2 months (T 2 ), and at the end of anti-TB treatment (T end ). We measured the levels of Interferon (IFN)-γ, interleukin (IL)-2, IL-12, IL-10, IL-13 and tumor necrosis factor (TNF)-α in supernatants collected from the QuantiFERON-TB Gold In-Tube assay (QFT-GIT), as well as the WBC, neutrophil, platelet count and neutrophil to lymphocyte ratio (NLR) in whole blood. Compared with baseline levels, WBC, neutrophil, and platelet counts were significantly lower following treatment. In addition, the NLR after treatment significantly decreased compared with baseline, whereas the IL-2/IFN-γ ratio increased after treatment. In conclusion, the levels of IL-2/IFN-γ ratios in the supernatant and the NLR might be useful biomarkers to evaluate the effectiveness of drug therapy in active TB patients.

Original languageEnglish
Article number1159
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Neutrophils
Cytokines
Antigens
Interferons
Interleukin-2
Lymphocytes
Platelet Count
Therapeutics
Drug Therapy
Interleukin-13
Interleukin-12
Infection Control
Gold
Interleukin-10
Tumor Necrosis Factor-alpha
Biomarkers

All Science Journal Classification (ASJC) codes

  • General

Cite this

Leem, Ah Young ; Song, Joo Han ; Lee, Eun Hye ; Lee, Hyejon ; Sim, Bora ; Kim, Song Yee ; Chung, Kyung Soo ; Kim, Eun Young ; Jung, Ji Ye ; Park, Moo Suk ; Kim, Young Sam ; Chang, Joon ; kang, youngae. / Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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abstract = "Multiple cytokines and inflammatory markers control TB infection. We aimed to investigate the changes in multiple cytokines and inflammatory markers in active TB patients following anti-TB drug therapy. Twenty-nine patients with active TB were recruited prospectively between December 2010 and July 2017. Blood samples were collected before (T 0 ), after 2 months (T 2 ), and at the end of anti-TB treatment (T end ). We measured the levels of Interferon (IFN)-γ, interleukin (IL)-2, IL-12, IL-10, IL-13 and tumor necrosis factor (TNF)-α in supernatants collected from the QuantiFERON-TB Gold In-Tube assay (QFT-GIT), as well as the WBC, neutrophil, platelet count and neutrophil to lymphocyte ratio (NLR) in whole blood. Compared with baseline levels, WBC, neutrophil, and platelet counts were significantly lower following treatment. In addition, the NLR after treatment significantly decreased compared with baseline, whereas the IL-2/IFN-γ ratio increased after treatment. In conclusion, the levels of IL-2/IFN-γ ratios in the supernatant and the NLR might be useful biomarkers to evaluate the effectiveness of drug therapy in active TB patients.",
author = "Leem, {Ah Young} and Song, {Joo Han} and Lee, {Eun Hye} and Hyejon Lee and Bora Sim and Kim, {Song Yee} and Chung, {Kyung Soo} and Kim, {Eun Young} and Jung, {Ji Ye} and Park, {Moo Suk} and Kim, {Young Sam} and Joon Chang and youngae kang",
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Leem, AY, Song, JH, Lee, EH, Lee, H, Sim, B, Kim, SY, Chung, KS, Kim, EY, Jung, JY, Park, MS, Kim, YS, Chang, J & kang, Y 2018, 'Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy', Scientific reports, vol. 8, no. 1, 1159. https://doi.org/10.1038/s41598-018-19523-7

Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy. / Leem, Ah Young; Song, Joo Han; Lee, Eun Hye; Lee, Hyejon; Sim, Bora; Kim, Song Yee; Chung, Kyung Soo; Kim, Eun Young; Jung, Ji Ye; Park, Moo Suk; Kim, Young Sam; Chang, Joon; kang, youngae.

In: Scientific reports, Vol. 8, No. 1, 1159, 01.12.2018.

Research output: Contribution to journalArticle

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AU - Leem, Ah Young

AU - Song, Joo Han

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AU - Lee, Hyejon

AU - Sim, Bora

AU - Kim, Song Yee

AU - Chung, Kyung Soo

AU - Kim, Eun Young

AU - Jung, Ji Ye

AU - Park, Moo Suk

AU - Kim, Young Sam

AU - Chang, Joon

AU - kang, youngae

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N2 - Multiple cytokines and inflammatory markers control TB infection. We aimed to investigate the changes in multiple cytokines and inflammatory markers in active TB patients following anti-TB drug therapy. Twenty-nine patients with active TB were recruited prospectively between December 2010 and July 2017. Blood samples were collected before (T 0 ), after 2 months (T 2 ), and at the end of anti-TB treatment (T end ). We measured the levels of Interferon (IFN)-γ, interleukin (IL)-2, IL-12, IL-10, IL-13 and tumor necrosis factor (TNF)-α in supernatants collected from the QuantiFERON-TB Gold In-Tube assay (QFT-GIT), as well as the WBC, neutrophil, platelet count and neutrophil to lymphocyte ratio (NLR) in whole blood. Compared with baseline levels, WBC, neutrophil, and platelet counts were significantly lower following treatment. In addition, the NLR after treatment significantly decreased compared with baseline, whereas the IL-2/IFN-γ ratio increased after treatment. In conclusion, the levels of IL-2/IFN-γ ratios in the supernatant and the NLR might be useful biomarkers to evaluate the effectiveness of drug therapy in active TB patients.

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