Abstract
Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.
| Original language | English |
|---|---|
| Pages (from-to) | 459-471 |
| Number of pages | 13 |
| Journal | Yonsei medical journal |
| Volume | 49 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 Jun 1 |
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All Science Journal Classification (ASJC) codes
- Medicine(all)
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Changes in inward rectifier K+ channels in hepatic stellate cells during primary culture. / Lee, Dong Hyeon; Kong, In Deok; Lee, Joong Woo; Park, Kyu Sang.
In: Yonsei medical journal, Vol. 49, No. 3, 01.06.2008, p. 459-471.Research output: Contribution to journal › Article
TY - JOUR
T1 - Changes in inward rectifier K+ channels in hepatic stellate cells during primary culture
AU - Lee, Dong Hyeon
AU - Kong, In Deok
AU - Lee, Joong Woo
AU - Park, Kyu Sang
PY - 2008/6/1
Y1 - 2008/6/1
N2 - Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.
AB - Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.
UR - http://www.scopus.com/inward/record.url?scp=48649083950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=48649083950&partnerID=8YFLogxK
U2 - 10.3349/ymj.2008.49.3.459
DO - 10.3349/ymj.2008.49.3.459
M3 - Article
C2 - 18581597
AN - SCOPUS:48649083950
VL - 49
SP - 459
EP - 471
JO - Yonsei Medical Journal
JF - Yonsei Medical Journal
SN - 0513-5796
IS - 3
ER -