Changes in inward rectifier K+ channels in hepatic stellate cells during primary culture

Dong Hyeon Lee, In Deok Kong, Joong Woo Lee, Kyu Sang Park

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.

Original languageEnglish
Pages (from-to)459-471
Number of pages13
JournalYonsei medical journal
Volume49
Issue number3
DOIs
Publication statusPublished - 2008 Jun 1

Fingerprint

Inwardly Rectifying Potassium Channel
Hepatic Stellate Cells
Membrane Potentials
Gramicidin
Patch-Clamp Techniques
Real-Time Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{a934390a30ab481ca8d475f8bb3dfb71,
title = "Changes in inward rectifier K+ channels in hepatic stellate cells during primary culture",
abstract = "Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.",
author = "Lee, {Dong Hyeon} and Kong, {In Deok} and Lee, {Joong Woo} and Park, {Kyu Sang}",
year = "2008",
month = "6",
day = "1",
doi = "10.3349/ymj.2008.49.3.459",
language = "English",
volume = "49",
pages = "459--471",
journal = "Yonsei Medical Journal",
issn = "0513-5796",
publisher = "Yonsei University College of Medicine",
number = "3",

}

Changes in inward rectifier K+ channels in hepatic stellate cells during primary culture. / Lee, Dong Hyeon; Kong, In Deok; Lee, Joong Woo; Park, Kyu Sang.

In: Yonsei medical journal, Vol. 49, No. 3, 01.06.2008, p. 459-471.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Changes in inward rectifier K+ channels in hepatic stellate cells during primary culture

AU - Lee, Dong Hyeon

AU - Kong, In Deok

AU - Lee, Joong Woo

AU - Park, Kyu Sang

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.

AB - Purpose: This study examined the expression and function of inward rectifier K+ channels in cultured rat hepatic stellate cells (HSC). Materials and Methods: The expression of inward rectifier K- channels was measured using real-time RT-PCR, and electrophysiological properties were determined using the gramicidin-perforated patch-clamp technique. Results: The dominant inward rectifier K+ channel subtypes were Kir2.1 and Kir6.1. These dominant K+ channel subtypes decreased significantly during the primary culture throughout activation process. HSC can be classified into two subgroups: one with an inward-rectifying K+ current (type 1) and the other without (type 2). The inward current was blocked by Ba2+ (100 μM) and enhanced by high K- (140 mM), more prominently in type 1 HSC. There was a correlation between the amplitude of the Ba2--sensitive current and the membrane potential. In addition, Ba2+ (300 μM) depolarized the membrane potential. After the culture period, the amplitude of the inward current decreased and the membrane potential became depolarized. Conclusion: HSC express inward rectifier K+ channels, which physiologically regulate membrane potential and decrease during the activation process. These results will potentially help determine properties of the inward rectifier K+ channels in HSC as well as their roles in the activation process.

UR - http://www.scopus.com/inward/record.url?scp=48649083950&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48649083950&partnerID=8YFLogxK

U2 - 10.3349/ymj.2008.49.3.459

DO - 10.3349/ymj.2008.49.3.459

M3 - Article

C2 - 18581597

AN - SCOPUS:48649083950

VL - 49

SP - 459

EP - 471

JO - Yonsei Medical Journal

JF - Yonsei Medical Journal

SN - 0513-5796

IS - 3

ER -