Changes in performance and bacterial communities in response to various process disturbances in a high-rate biohydrogen reactor fed with galactose

Jeong Hoon Park, Gopalakrishnan Kumar, Jong Hun Park, Hee Deung Park, Sang Hyoun Kim

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

High-rate biohydrogen production was achieved via hybrid immobilized cells fed with galactose in a continuous reactor system. The hybrid immobilized cells were broken down after 20days and began to form granules by self-aggregation. The peak hydrogen production rate (HPR) and hydrogen yield (HY) of 11.8±0.6LH2/L-d and 2.1±0.1molH2/molgalactoseadded, respectively, were achieved at the hydraulic retention time (HRT) of 8h with an organic loading rate (OLR) of 45g/L-d. This is the highest yet reported for the employment of galactose in a continuous system. Various process disturbances including shock loading, acidification, alkalization and starvation were examined through bacterial community analysis via pyrosequencing of the 16S rRNA genes. The proportion of Clostridia increased during the stable biohydrogen production periods, while that of Bacilli increased when the reactor was disturbed. However, due to the stability of the self-aggregated granules, the process performance was regained within 4-7days.

Original languageEnglish
Pages (from-to)109-116
Number of pages8
JournalBioresource Technology
Volume188
DOIs
Publication statusPublished - 2015 Jul 1

Fingerprint

Galactose
Cells
disturbance
Clostridium
Acidification
Bacilli
Hydrogen production
hydrogen
Hydrogen
Agglomeration
Genes
Hydraulics
starvation
acidification
hydraulics
gene
reactor
rate

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

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abstract = "High-rate biohydrogen production was achieved via hybrid immobilized cells fed with galactose in a continuous reactor system. The hybrid immobilized cells were broken down after 20days and began to form granules by self-aggregation. The peak hydrogen production rate (HPR) and hydrogen yield (HY) of 11.8±0.6LH2/L-d and 2.1±0.1molH2/molgalactoseadded, respectively, were achieved at the hydraulic retention time (HRT) of 8h with an organic loading rate (OLR) of 45g/L-d. This is the highest yet reported for the employment of galactose in a continuous system. Various process disturbances including shock loading, acidification, alkalization and starvation were examined through bacterial community analysis via pyrosequencing of the 16S rRNA genes. The proportion of Clostridia increased during the stable biohydrogen production periods, while that of Bacilli increased when the reactor was disturbed. However, due to the stability of the self-aggregated granules, the process performance was regained within 4-7days.",
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Changes in performance and bacterial communities in response to various process disturbances in a high-rate biohydrogen reactor fed with galactose. / Park, Jeong Hoon; Kumar, Gopalakrishnan; Park, Jong Hun; Park, Hee Deung; Kim, Sang Hyoun.

In: Bioresource Technology, Vol. 188, 01.07.2015, p. 109-116.

Research output: Contribution to journalArticle

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AU - Kim, Sang Hyoun

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