Channel surface patterning of alternating biomimetic protein combinations for enhanced microfluidic tumor cell isolation

Cari Launiere, Marissa Gaskill, Gregory Czaplewski, Ja Hye Myung, Seungpyo Hong, David T. Eddington

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Here, we report a new method for multicomponent protein patterning in a microchannel and also a technique for improving immunoaffinity-based circulating tumor cell (CTC) capture by patterning regions of alternating adhesive proteins using the new method. The first of two proteins, antiepithelial cell adhesion molecule (anti-EpCAM), provides the specificity for CTC capture. The second, E-selectin, increases CTC capture under shear. Patterning regions with and without E-selectin allows captured leukocytes, which also bind E-selectin and are unwanted impurities in CTC isolation, to roll a short distance and detach from the capture surface. This reduces leukocyte capture by up to 82%. The patterning is combined with a leukocyte elution step in which a calcium chelating buffer effectively deactivates E-selectin so that leukocytes may be rinsed away 60% more efficiently than with a buffer containing calcium. The alternating patterning of this biomimetic protein combination, used in conjunction with the elution step, reduces capture of leukocytes while maintaining a high tumor cell capture efficiency that is up to 1.9 times higher than the tumor cell capture efficiency of a surface with only anti-EpCAM. The new patterning technique described here does not require mask alignment and can be used to spatially control the immobilization of any two proteins or protein mixtures inside a sealed microfluidic channel.

Original languageEnglish
Pages (from-to)4022-4028
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number9
DOIs
Publication statusPublished - 2012 May 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this