Chaperone-like protein DAY plays critical roles in photomorphogenesis

Ho Seok Lee, Ilyeong Choi, Young Jeon, Hee Kyung Ahn, Huikyong Cho, Ji Woo Kim, Jae Hee Kim, Jung Min Lee, Sung Hee Lee, Julian Bünting, Dong Hye Seo, Tak Lee, Du Hwa Lee, Insuk Lee, Man Ho Oh, Tae Wuk Kim, Youssef Belkhadir, Hyun Sook Pai

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARKANDYELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments.

Original languageEnglish
Article number4194
JournalNature communications
Issue number1
Publication statusPublished - 2021 Dec 1

Bibliographical note

Funding Information:
This work was supported by grants from the Austrian Academy of Science through the Gregor Mendel Institute and by the Austrian Science Fund (FWF) (I 3654) to Y.B. This research was supported by the following grants to HSP: Basic Science Research Program (NRF-2018R1A6A1A03025607) and Mid-Career Researcher Program (NRF-2019R1A2B5B01069573) from the National Research Foundation (NRF) of the Republic of Korea. The research was supported in part by Brain Korea 21 (BK21) FOUR program; I.Y.C. is a fellowship awardee of BK21 FOUR program.

Publisher Copyright:
© 2021, The Author(s).

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)


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