Chaperone-like protein DAY plays critical roles in photomorphogenesis

Ho Seok Lee; Ilyeong Choi; Young Jeon; Hee Kyung Ahn; Huikyong Cho; Ji Woo Kim; Jae Hee Kim; Jung Min Lee; Sung Hee Lee; Julian Bünting; Dong Hye Seo; Tak Lee; Du Hwa Lee; Insuk Lee; Man Ho Oh; Tae Wuk Kim; Youssef Belkhadir; Hyun Sook Pai

doi:10.1038/s41467-021-24446-5

Chaperone-like protein DAY plays critical roles in photomorphogenesis

Ho Seok Lee, Ilyeong Choi, Young Jeon, Hee Kyung Ahn, Huikyong Cho, Ji Woo Kim, Jae Hee Kim, Jung Min Lee, Sung Hee Lee, Julian Bünting, Dong Hye Seo, Tak Lee, Du Hwa Lee, Insuk Lee, Man Ho Oh, Tae Wuk Kim, Youssef Belkhadir, Hyun Sook Pai

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARKANDYELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments.

Original language	English
Article number	4194
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-24446-5
Publication status	Published - 2021 Dec 1

Bibliographical note

Funding Information:
This work was supported by grants from the Austrian Academy of Science through the Gregor Mendel Institute and by the Austrian Science Fund (FWF) (I 3654) to Y.B. This research was supported by the following grants to HSP: Basic Science Research Program (NRF-2018R1A6A1A03025607) and Mid-Career Researcher Program (NRF-2019R1A2B5B01069573) from the National Research Foundation (NRF) of the Republic of Korea. The research was supported in part by Brain Korea 21 (BK21) FOUR program; I.Y.C. is a fellowship awardee of BK21 FOUR program.

Publisher Copyright:
© 2021, The Author(s).

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/s41467-021-24446-5

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title = "Chaperone-like protein DAY plays critical roles in photomorphogenesis",

abstract = "Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARKANDYELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments.",

author = "Lee, {Ho Seok} and Ilyeong Choi and Young Jeon and Ahn, {Hee Kyung} and Huikyong Cho and Kim, {Ji Woo} and Kim, {Jae Hee} and Lee, {Jung Min} and Lee, {Sung Hee} and Julian B{\"u}nting and Seo, {Dong Hye} and Tak Lee and Lee, {Du Hwa} and Insuk Lee and Oh, {Man Ho} and Kim, {Tae Wuk} and Youssef Belkhadir and Pai, {Hyun Sook}",

note = "Funding Information: This work was supported by grants from the Austrian Academy of Science through the Gregor Mendel Institute and by the Austrian Science Fund (FWF) (I 3654) to Y.B. This research was supported by the following grants to HSP: Basic Science Research Program (NRF-2018R1A6A1A03025607) and Mid-Career Researcher Program (NRF-2019R1A2B5B01069573) from the National Research Foundation (NRF) of the Republic of Korea. The research was supported in part by Brain Korea 21 (BK21) FOUR program; I.Y.C. is a fellowship awardee of BK21 FOUR program. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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AU - Lee, Ho Seok

AU - Choi, Ilyeong

AU - Jeon, Young

AU - Ahn, Hee Kyung

AU - Cho, Huikyong

AU - Kim, Ji Woo

AU - Kim, Jae Hee

AU - Lee, Jung Min

AU - Lee, Sung Hee

AU - Bünting, Julian

AU - Seo, Dong Hye

AU - Lee, Tak

AU - Lee, Du Hwa

AU - Lee, Insuk

AU - Oh, Man Ho

AU - Kim, Tae Wuk

AU - Belkhadir, Youssef

AU - Pai, Hyun Sook

N1 - Funding Information: This work was supported by grants from the Austrian Academy of Science through the Gregor Mendel Institute and by the Austrian Science Fund (FWF) (I 3654) to Y.B. This research was supported by the following grants to HSP: Basic Science Research Program (NRF-2018R1A6A1A03025607) and Mid-Career Researcher Program (NRF-2019R1A2B5B01069573) from the National Research Foundation (NRF) of the Republic of Korea. The research was supported in part by Brain Korea 21 (BK21) FOUR program; I.Y.C. is a fellowship awardee of BK21 FOUR program. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Photomorphogenesis, light-mediated development, is an essential feature of all terrestrial plants. While chloroplast development and brassinosteroid (BR) signaling are known players in photomorphogenesis, proteins that regulate both pathways have yet to be identified. Here we report that DE-ETIOLATION IN THE DARKANDYELLOWING IN THE LIGHT (DAY), a membrane protein containing DnaJ-like domain, plays a dual-role in photomorphogenesis by stabilizing the BR receptor, BRI1, as well as a key enzyme in chlorophyll biosynthesis, POR. DAY localizes to both the endomembrane and chloroplasts via its first transmembrane domain and chloroplast transit peptide, respectively, and interacts with BRI1 and POR in their respective subcellular compartments. Using genetic analysis, we show that DAY acts independently on BR signaling and chlorophyll biogenesis. Collectively, this work uncovers DAY as a factor that simultaneously regulates BR signaling and chloroplast development, revealing a key regulator of photomorphogenesis that acts across cell compartments.

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Chaperone-like protein DAY plays critical roles in photomorphogenesis

Abstract

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