Inhibitor of apoptosis (IAP) proteins are a family of evolutionarily conserved proteins that regulate apoptosis as well as other cellular processes. The functions of many IAPs are defined by their RING domains, which possess E3 ubiquitin ligase activity and promote proteasomal degradation of an increasing number of target proteins. In this chapter, we describe the methods used in our laboratories to study the IAP's E3 activity.
|Title of host publication||Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B|
|Editors||Roya Khosravi-Far, Zahra Zakeri, Richard Lockshin, Mauro Piacentini|
|Number of pages||11|
|Publication status||Published - 2008 Sep 24|
|Name||Methods in Enzymology|
All Science Journal Classification (ASJC) codes
- Molecular Biology