Inhibitor of apoptosis (IAP) proteins are a family of evolutionarily conserved proteins that regulate apoptosis as well as other cellular processes. The functions of many IAPs are defined by their RING domains, which possess E3 ubiquitin ligase activity and promote proteasomal degradation of an increasing number of target proteins. In this chapter, we describe the methods used in our laboratories to study the IAP's E3 activity.
|Title of host publication||Programmed Cell Death, The Biology and Therapeutic Implications of Cell Death, Part B|
|Editors||Roya Khosravi-Far, Zahra Zakeri, Richard Lockshin, Mauro Piacentini|
|Number of pages||11|
|Publication status||Published - 2008|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
This work was supported by NIH grants CA88868 and CA108872 and a Leukemia & Lymphoma Society Scholar award to X. Y., and the Korea Research Foundation Grant (KRF‐2006‐005‐J04502) and Brain Korea 21 project to T.H.L. The authors are grateful for critical reading of the manuscript by the members of the Yang laboratory.
All Science Journal Classification (ASJC) codes
- Molecular Biology