Chapter 17 Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo

Hee Jun Na, Hun Taeg Chung, Kwon Soo Ha, Hansoo Lee, Young-Guen Kwon, Timothy R. Billiar, Young Myeong Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)

Abstract

Nitrosative stress, a nitric oxide (NO)-mediated nitrosylation of redox-sensitive thiols, has been linked to the regulation of signal transduction, gene expression, and cell growth and apoptosis and thus may be widely implicated in both physiological and pathological actions of NO. Protein S-nitrosylation has been observed to occur in vitro and in vivo in pathophysiological conditions. Apoptosis can be regulated by S-nitrosylation of the redox-sensitive cysteine residue in the active site of all caspase family proteases. Detection and measurement for the modification and inactivation of caspases by S-nitrosylation remain a new challenge because of the lability of the S-nitrosothiol moiety. This chapter describes approaches for assaying and identifying S-nitrosylated caspase enzymes in vitro and in vivo. These methods permit rapid and reproducible assays of S-nitrosylated caspases in biological and clinical specimens and should be useful for studies defining a pathophysiological role of NO in several apoptosis-associated human diseases.

Original languageEnglish
Title of host publicationNitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling
PublisherAcademic Press Inc.
Pages317-327
Number of pages11
ISBN (Print)9780123743091
DOIs
Publication statusPublished - 2008 Jan 1

Publication series

NameMethods in Enzymology
Volume441
ISSN (Print)0076-6879

Fingerprint

Caspases
Nitric Oxide
Apoptosis
Oxidation-Reduction
S-Nitrosothiols
Signal transduction
Protein S
Cell growth
Sulfhydryl Compounds
Gene expression
Cysteine
Assays
Signal Transduction
Catalytic Domain
Peptide Hydrolases
Gene Expression
In Vitro Techniques
Enzymes
Growth

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Na, H. J., Chung, H. T., Ha, K. S., Lee, H., Kwon, Y-G., Billiar, T. R., & Kim, Y. M. (2008). Chapter 17 Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo. In Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling (pp. 317-327). (Methods in Enzymology; Vol. 441). Academic Press Inc.. https://doi.org/10.1016/S0076-6879(08)01217-2
Na, Hee Jun ; Chung, Hun Taeg ; Ha, Kwon Soo ; Lee, Hansoo ; Kwon, Young-Guen ; Billiar, Timothy R. ; Kim, Young Myeong. / Chapter 17 Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo. Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling. Academic Press Inc., 2008. pp. 317-327 (Methods in Enzymology).
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Na, HJ, Chung, HT, Ha, KS, Lee, H, Kwon, Y-G, Billiar, TR & Kim, YM 2008, Chapter 17 Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo. in Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling. Methods in Enzymology, vol. 441, Academic Press Inc., pp. 317-327. https://doi.org/10.1016/S0076-6879(08)01217-2

Chapter 17 Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo. / Na, Hee Jun; Chung, Hun Taeg; Ha, Kwon Soo; Lee, Hansoo; Kwon, Young-Guen; Billiar, Timothy R.; Kim, Young Myeong.

Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling. Academic Press Inc., 2008. p. 317-327 (Methods in Enzymology; Vol. 441).

Research output: Chapter in Book/Report/Conference proceedingChapter

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AU - Billiar, Timothy R.

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N2 - Nitrosative stress, a nitric oxide (NO)-mediated nitrosylation of redox-sensitive thiols, has been linked to the regulation of signal transduction, gene expression, and cell growth and apoptosis and thus may be widely implicated in both physiological and pathological actions of NO. Protein S-nitrosylation has been observed to occur in vitro and in vivo in pathophysiological conditions. Apoptosis can be regulated by S-nitrosylation of the redox-sensitive cysteine residue in the active site of all caspase family proteases. Detection and measurement for the modification and inactivation of caspases by S-nitrosylation remain a new challenge because of the lability of the S-nitrosothiol moiety. This chapter describes approaches for assaying and identifying S-nitrosylated caspase enzymes in vitro and in vivo. These methods permit rapid and reproducible assays of S-nitrosylated caspases in biological and clinical specimens and should be useful for studies defining a pathophysiological role of NO in several apoptosis-associated human diseases.

AB - Nitrosative stress, a nitric oxide (NO)-mediated nitrosylation of redox-sensitive thiols, has been linked to the regulation of signal transduction, gene expression, and cell growth and apoptosis and thus may be widely implicated in both physiological and pathological actions of NO. Protein S-nitrosylation has been observed to occur in vitro and in vivo in pathophysiological conditions. Apoptosis can be regulated by S-nitrosylation of the redox-sensitive cysteine residue in the active site of all caspase family proteases. Detection and measurement for the modification and inactivation of caspases by S-nitrosylation remain a new challenge because of the lability of the S-nitrosothiol moiety. This chapter describes approaches for assaying and identifying S-nitrosylated caspase enzymes in vitro and in vivo. These methods permit rapid and reproducible assays of S-nitrosylated caspases in biological and clinical specimens and should be useful for studies defining a pathophysiological role of NO in several apoptosis-associated human diseases.

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BT - Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling

PB - Academic Press Inc.

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Na HJ, Chung HT, Ha KS, Lee H, Kwon Y-G, Billiar TR et al. Chapter 17 Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo. In Nitric Oxide, Part G Oxidative and Nitrosative Stress in Redox Regulation of Cell Signaling. Academic Press Inc. 2008. p. 317-327. (Methods in Enzymology). https://doi.org/10.1016/S0076-6879(08)01217-2