Characteristics and Responses of Human Vocal Fold Cells in a Vibrational Culture Model

Dongjoo Kim, Songyi Lee, Jae Yol Lim, Soonjo Kwon

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Objectives/Hypothesis: This study was conducted to provide a vibrational culture model to investigate the effects of mechanical environments on cellular functions, and elucidate physiological characteristics of two different types of cells in vocal folds under static and vibrational conditions. Study Design: In vitro study of human vocal fold fibroblasts (hVFFs) and human macula flava stellate cells (hMF-SCs). Methods: hVFFs and hMF-SCs were exposed to a 2-second-on/2-second-off, 205 Hz vibration regime for 4 hours by using a vibrational culture model. We compared cell morphology, cell viability, and gene expression in extracellular matrix–related components, growth factors, and differentiation markers under static and vibratory conditions. Results: hVFFs and hMF-SCs differed in their morphologies and gene expression levels under static condition. The applied vibration did not induce changes in morphology and viability of either cell type. However, gene expression levels changed in both cell types in response to vibration; in particular, hMF-SCs exhibited a more sensitive response to vibration than that shown by hVFFs. Conclusions: The vibrational culture model developed in this study enabled us to investigate the effects of the applied vibration on two types of vocal fold resident cells. As a result, we could demonstrate that hVFFs and hMF-SCs exhibited distinctively different characteristics under vibrational conditions. Level of Evidence: NA. Laryngoscope, 128:E258–E264, 2018.

Original languageEnglish
Pages (from-to)E258-E264
Issue number7
Publication statusPublished - 2018 Jul

Bibliographical note

Funding Information:
Funding was provided by an Inha University Research Grant, National Research Foundation of Korea (NRF-2017R1D1A1B03029589), and the Korea Ministry of Environment as the Environmental Health Action Program (RE201603084).

Publisher Copyright:
© 2018 The American Laryngological, Rhinological and Otological Society, Inc.

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology


Dive into the research topics of 'Characteristics and Responses of Human Vocal Fold Cells in a Vibrational Culture Model'. Together they form a unique fingerprint.

Cite this