Characteristics and Responses of Human Vocal Fold Cells in a Vibrational Culture Model

Dongjoo Kim, Songyi Lee, JaeYoul Lim, Soonjo Kwon

Research output: Contribution to journalArticle

Abstract

Objectives/Hypothesis: This study was conducted to provide a vibrational culture model to investigate the effects of mechanical environments on cellular functions, and elucidate physiological characteristics of two different types of cells in vocal folds under static and vibrational conditions. Study Design: In vitro study of human vocal fold fibroblasts (hVFFs) and human macula flava stellate cells (hMF-SCs). Methods: hVFFs and hMF-SCs were exposed to a 2-second-on/2-second-off, 205 Hz vibration regime for 4 hours by using a vibrational culture model. We compared cell morphology, cell viability, and gene expression in extracellular matrix–related components, growth factors, and differentiation markers under static and vibratory conditions. Results: hVFFs and hMF-SCs differed in their morphologies and gene expression levels under static condition. The applied vibration did not induce changes in morphology and viability of either cell type. However, gene expression levels changed in both cell types in response to vibration; in particular, hMF-SCs exhibited a more sensitive response to vibration than that shown by hVFFs. Conclusions: The vibrational culture model developed in this study enabled us to investigate the effects of the applied vibration on two types of vocal fold resident cells. As a result, we could demonstrate that hVFFs and hMF-SCs exhibited distinctively different characteristics under vibrational conditions. Level of Evidence: NA. Laryngoscope, 128:E258–E264, 2018.

Original languageEnglish
Pages (from-to)E258-E264
JournalLaryngoscope
Volume128
Issue number7
DOIs
Publication statusPublished - 2018 Jul 1

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Vocal Cords
Vibration
Fibroblasts
Gene Expression
Cell Survival
Laryngoscopes
Differentiation Antigens
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Kim, Dongjoo ; Lee, Songyi ; Lim, JaeYoul ; Kwon, Soonjo. / Characteristics and Responses of Human Vocal Fold Cells in a Vibrational Culture Model. In: Laryngoscope. 2018 ; Vol. 128, No. 7. pp. E258-E264.
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abstract = "Objectives/Hypothesis: This study was conducted to provide a vibrational culture model to investigate the effects of mechanical environments on cellular functions, and elucidate physiological characteristics of two different types of cells in vocal folds under static and vibrational conditions. Study Design: In vitro study of human vocal fold fibroblasts (hVFFs) and human macula flava stellate cells (hMF-SCs). Methods: hVFFs and hMF-SCs were exposed to a 2-second-on/2-second-off, 205 Hz vibration regime for 4 hours by using a vibrational culture model. We compared cell morphology, cell viability, and gene expression in extracellular matrix–related components, growth factors, and differentiation markers under static and vibratory conditions. Results: hVFFs and hMF-SCs differed in their morphologies and gene expression levels under static condition. The applied vibration did not induce changes in morphology and viability of either cell type. However, gene expression levels changed in both cell types in response to vibration; in particular, hMF-SCs exhibited a more sensitive response to vibration than that shown by hVFFs. Conclusions: The vibrational culture model developed in this study enabled us to investigate the effects of the applied vibration on two types of vocal fold resident cells. As a result, we could demonstrate that hVFFs and hMF-SCs exhibited distinctively different characteristics under vibrational conditions. Level of Evidence: NA. Laryngoscope, 128:E258–E264, 2018.",
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Characteristics and Responses of Human Vocal Fold Cells in a Vibrational Culture Model. / Kim, Dongjoo; Lee, Songyi; Lim, JaeYoul; Kwon, Soonjo.

In: Laryngoscope, Vol. 128, No. 7, 01.07.2018, p. E258-E264.

Research output: Contribution to journalArticle

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