Characteristics of cellulase modified with amphiphilic copolymer in organic solvent

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Characteristics of modified cellulases in organic solvents were studied. Cellulases modified with amphiphilic copolymer of polyoxyalkylene (POA)-derivative and maleic acid anhydride (MAA). Amino groups of the cellulase molecule were easily coupled with the MAA functional group of the copolymer. At the maximum degree of modification (DM) of 55%, the activity of modified cellulase retained more than 80% of the unmodified native cellulase activity. The modified cellulase using AKM-1511 with DM greater than 40% was found to be more than 90% soluble in aqueous solution of acetone and ethanol, leaving the native cellulase and impurities in the fermentation broth with the residue. Modified cellulase showed excellent stability against water-insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer, which consists of ethylene oxide (EO) and propylene oxide (PO), could be dissolved in these solvents.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalKorean Journal of Chemical Engineering
Volume12
Issue number5
DOIs
Publication statusPublished - 1995 Nov

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Fingerprint Dive into the research topics of 'Characteristics of cellulase modified with amphiphilic copolymer in organic solvent'. Together they form a unique fingerprint.

  • Cite this