Characteristics of cellulase modified with amphiphilic copolymer in organic solvent

Research output: Contribution to journalArticle

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Abstract

Characteristics of modified cellulases in organic solvents were studied. Cellulases modified with amphiphilic copolymer of polyoxyalkylene (POA)-derivative and maleic acid anhydride (MAA). Amino groups of the cellulase molecule were easily coupled with the MAA functional group of the copolymer. At the maximum degree of modification (DM) of 55%, the activity of modified cellulase retained more than 80% of the unmodified native cellulase activity. The modified cellulase using AKM-1511 with DM greater than 40% was found to be more than 90% soluble in aqueous solution of acetone and ethanol, leaving the native cellulase and impurities in the fermentation broth with the residue. Modified cellulase showed excellent stability against water-insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer, which consists of ethylene oxide (EO) and propylene oxide (PO), could be dissolved in these solvents.

Original languageEnglish
Pages (from-to)523-527
Number of pages5
JournalKorean Journal of Chemical Engineering
Volume12
Issue number5
DOIs
Publication statusPublished - 1995 Nov 1

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Cellulase
Organic solvents
Copolymers
Oxides
Acids
Cellulases
Anhydrides
Acetone
Fermentation
Functional groups
Propylene
Ethylene
Ethanol
Impurities
Derivatives
Molecules
Ethylene Oxide
Water

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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title = "Characteristics of cellulase modified with amphiphilic copolymer in organic solvent",
abstract = "Characteristics of modified cellulases in organic solvents were studied. Cellulases modified with amphiphilic copolymer of polyoxyalkylene (POA)-derivative and maleic acid anhydride (MAA). Amino groups of the cellulase molecule were easily coupled with the MAA functional group of the copolymer. At the maximum degree of modification (DM) of 55{\%}, the activity of modified cellulase retained more than 80{\%} of the unmodified native cellulase activity. The modified cellulase using AKM-1511 with DM greater than 40{\%} was found to be more than 90{\%} soluble in aqueous solution of acetone and ethanol, leaving the native cellulase and impurities in the fermentation broth with the residue. Modified cellulase showed excellent stability against water-insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer, which consists of ethylene oxide (EO) and propylene oxide (PO), could be dissolved in these solvents.",
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Characteristics of cellulase modified with amphiphilic copolymer in organic solvent. / Park, Jin Won.

In: Korean Journal of Chemical Engineering, Vol. 12, No. 5, 01.11.1995, p. 523-527.

Research output: Contribution to journalArticle

TY - JOUR

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N2 - Characteristics of modified cellulases in organic solvents were studied. Cellulases modified with amphiphilic copolymer of polyoxyalkylene (POA)-derivative and maleic acid anhydride (MAA). Amino groups of the cellulase molecule were easily coupled with the MAA functional group of the copolymer. At the maximum degree of modification (DM) of 55%, the activity of modified cellulase retained more than 80% of the unmodified native cellulase activity. The modified cellulase using AKM-1511 with DM greater than 40% was found to be more than 90% soluble in aqueous solution of acetone and ethanol, leaving the native cellulase and impurities in the fermentation broth with the residue. Modified cellulase showed excellent stability against water-insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer, which consists of ethylene oxide (EO) and propylene oxide (PO), could be dissolved in these solvents.

AB - Characteristics of modified cellulases in organic solvents were studied. Cellulases modified with amphiphilic copolymer of polyoxyalkylene (POA)-derivative and maleic acid anhydride (MAA). Amino groups of the cellulase molecule were easily coupled with the MAA functional group of the copolymer. At the maximum degree of modification (DM) of 55%, the activity of modified cellulase retained more than 80% of the unmodified native cellulase activity. The modified cellulase using AKM-1511 with DM greater than 40% was found to be more than 90% soluble in aqueous solution of acetone and ethanol, leaving the native cellulase and impurities in the fermentation broth with the residue. Modified cellulase showed excellent stability against water-insoluble solvent. Moreover, cellulase modified with hydrophobic copolymer, which consists of ethylene oxide (EO) and propylene oxide (PO), could be dissolved in these solvents.

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