Characteristics of ethylnitrosourea-induced cataracts

Min Jung Lee, Jae Young Kim, Yeun Jung Kim, Jae Woo Cho, Kyu Hyuk Cho, Chang Woo Song, Han Sung Jung

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Abstract

Purpose: This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse. Materials and Methods: Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract. Results: A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract. Conclusions: Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalCurrent Eye Research
Volume34
Issue number5
DOIs
Publication statusPublished - 2009 May

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Lee, M. J., Kim, J. Y., Kim, Y. J., Cho, J. W., Cho, K. H., Song, C. W., & Jung, H. S. (2009). Characteristics of ethylnitrosourea-induced cataracts. Current Eye Research, 34(5), 360-368. https://doi.org/10.1080/02713680902843047