Characteristics of intracellular algogenic organic matter and its reactivity with hydroxyl radicals

Doorae Lee, Minhwan Kwon, Yongtae Ahn, Youmi Jung, Seong Nam Nam, Il hwan Choi, Joonwun Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to investigate the reactivity of intracellular algogenic organic matter (IOM) with hydroxyl radicals (·OH), a key reaction species in advanced oxidation processes. IOM was extracted from two green algae, Chlamydomonas reinhardtii and Scenedesmus sp., and two blue-green algae, Anabaena sp. and Microcystis aeruginosa using a freeze–thaw method. The second-order rate constants of the extracted IOM with ·OH were determined as 7.95 × 10 8 M C −1 s −1 (Chlamydomonas reinhardtii), 6.71 × 10 8 M C −1 s −1 (Scenedesmus sp.), 4.02 × 10 8 M C −1 s −1 (Anabaena sp.), and 4.45 × 10 8 M C −1 s −1 (Microcystis aeruginosa). These rate constants were significantly higher than values reported for dissolved organic matter in various water sources. This implies that IOM formation during algal bloom season could change the ·OH water matrix demand and adversely affect the performance of advanced oxidation processes. To investigate the physical and chemical composition characteristics of IOM and their relationship to the rate constants determined for the reaction between IOM and ·OH, liquid chromatography-organic carbon detection (LC-OCD) and fluorescence excitation-emission matrix & parallel factor analysis (FEEM-PARAFAC) were used. The IOM mainly consisted of low molecular weight (LMW) matter and protein-related compounds, as evidenced LMW neutrals (38–65%), biopolymers (7–19%), and tryptophan-like compounds (74–94%). Based on the composition characteristics of IOM, it was concluded that the molecular weight and the presence of nitrogen-containing compounds are influential parameters for determining the reactivity of IOM with ·OH.

Original languageEnglish
Pages (from-to)13-25
Number of pages13
JournalWater Research
Volume144
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

hydroxyl radical
Biological materials
organic matter
Rate constants
Molecular weight
oxidation
matrix
nitrogen compound
Oxidation
Biopolymers
Liquid chromatography
green alga
dissolved organic matter
Factor analysis
factor analysis
Algae
Organic carbon
Chemical analysis
liquid chromatography
cyanobacterium

All Science Journal Classification (ASJC) codes

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Lee, Doorae ; Kwon, Minhwan ; Ahn, Yongtae ; Jung, Youmi ; Nam, Seong Nam ; Choi, Il hwan ; Kang, Joonwun. / Characteristics of intracellular algogenic organic matter and its reactivity with hydroxyl radicals. In: Water Research. 2018 ; Vol. 144. pp. 13-25.
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abstract = "The aim of this study was to investigate the reactivity of intracellular algogenic organic matter (IOM) with hydroxyl radicals (·OH), a key reaction species in advanced oxidation processes. IOM was extracted from two green algae, Chlamydomonas reinhardtii and Scenedesmus sp., and two blue-green algae, Anabaena sp. and Microcystis aeruginosa using a freeze–thaw method. The second-order rate constants of the extracted IOM with ·OH were determined as 7.95 × 10 8 M C −1 s −1 (Chlamydomonas reinhardtii), 6.71 × 10 8 M C −1 s −1 (Scenedesmus sp.), 4.02 × 10 8 M C −1 s −1 (Anabaena sp.), and 4.45 × 10 8 M C −1 s −1 (Microcystis aeruginosa). These rate constants were significantly higher than values reported for dissolved organic matter in various water sources. This implies that IOM formation during algal bloom season could change the ·OH water matrix demand and adversely affect the performance of advanced oxidation processes. To investigate the physical and chemical composition characteristics of IOM and their relationship to the rate constants determined for the reaction between IOM and ·OH, liquid chromatography-organic carbon detection (LC-OCD) and fluorescence excitation-emission matrix & parallel factor analysis (FEEM-PARAFAC) were used. The IOM mainly consisted of low molecular weight (LMW) matter and protein-related compounds, as evidenced LMW neutrals (38–65{\%}), biopolymers (7–19{\%}), and tryptophan-like compounds (74–94{\%}). Based on the composition characteristics of IOM, it was concluded that the molecular weight and the presence of nitrogen-containing compounds are influential parameters for determining the reactivity of IOM with ·OH.",
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Characteristics of intracellular algogenic organic matter and its reactivity with hydroxyl radicals. / Lee, Doorae; Kwon, Minhwan; Ahn, Yongtae; Jung, Youmi; Nam, Seong Nam; Choi, Il hwan; Kang, Joonwun.

In: Water Research, Vol. 144, 01.11.2018, p. 13-25.

Research output: Contribution to journalArticle

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