Characterization and cDNA cloning of halyxin, a heterogeneous three-chain anticoagulant protein from the venom of Agkistrodon halys brevicaudus

Bon Hun Koo, Young Doug Sohn, Ki Chul Hwang, Yangsoo Jang, Doo Sik Kim, Kwang Hoe Chung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We report upon the isolation, characterization, and cDNA cloning of an anticoagulant protein, halyxin from Agkistrodon halys brevicaudus venom. The protein exists as a 29kDa protein, and is separated into three chains on SDS-PAGE under reducing conditions. However, we cloned only two cDNAs encoding halyxin from the cDNA library of the snake venom gland, on the basis of the determined amino acid sequences. The complete amino acid sequences were deduced from their nucleotide sequences and named halyxin A (129 amino acid residues) and B chain (123 amino acid residues). The deduced amino acid sequence of halyxin A chain corresponds to the two smaller chains. Thus, it is considered that halyxin A chain could be synthesized as a single-chain protein that is subsequently cleaved to yield the mature two-chain protein. The amino acid sequence of halyxin is similar to that of other snake venom proteins of the C-type lectin superfamily, and prolongs plasma-clotting time. In the presence of Ca2+ ions, halyxin binds to coagulation factors IX, X, IXa, and Xa, but not to other vitamin K-dependent coagulation factors. It also inhibits factor Xa in a non-competitive manner but does not affect other activated coagulation factors.

Original languageEnglish
Pages (from-to)947-957
Number of pages11
JournalToxicon
Volume40
Issue number7
DOIs
Publication statusPublished - 2002 Jan 1

Fingerprint

Cloning
Anticoagulants
Organism Cloning
Complementary DNA
Amino Acid Sequence
Amino Acids
Snake Venoms
Blood Coagulation Factors
Proteins
C-Type Lectins
Factor X
Factor Xa
Factor IX
Vitamin K
Venoms
Protein C
Gene Library
Polyacrylamide Gel Electrophoresis
Agkistrodon venoms
Ions

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Koo, Bon Hun ; Sohn, Young Doug ; Hwang, Ki Chul ; Jang, Yangsoo ; Kim, Doo Sik ; Chung, Kwang Hoe. / Characterization and cDNA cloning of halyxin, a heterogeneous three-chain anticoagulant protein from the venom of Agkistrodon halys brevicaudus. In: Toxicon. 2002 ; Vol. 40, No. 7. pp. 947-957.
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Characterization and cDNA cloning of halyxin, a heterogeneous three-chain anticoagulant protein from the venom of Agkistrodon halys brevicaudus. / Koo, Bon Hun; Sohn, Young Doug; Hwang, Ki Chul; Jang, Yangsoo; Kim, Doo Sik; Chung, Kwang Hoe.

In: Toxicon, Vol. 40, No. 7, 01.01.2002, p. 947-957.

Research output: Contribution to journalArticle

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