Characterization and stability studies of a novel liposomal cyclosporin a prepared using the supercritical fluid method

Comparison with the modified conventional Bangham method

Pankaj Ranjan Karn, Wonkyung Cho, Hee Jun Park, Jeong Sook Park, Sung Joo Hwang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A novel method to prepare cyclosporin A encapsulated liposomes was introduced using supercritical fluid of carbon dioxide (SCF-CO2) as an antisolvent. To investigate the strength of the newly developed SCF-CO2 method compared with the modified conventional Bangham method, particle size, zeta potential, and polydispersity index (PDI) of both liposomal formulations were characterized and compared. In addition, entrapment efficiency (EE) and drug loading (DL) characteristics were analyzed by reversed-phase high-performance liquid chromatography. Significantly larger particle size and PDI were revealed from the conventional method, while EE (%) and DL (%) did not exhibit any significant differences. The SCF-CO2 liposomes were found to be relatively smaller, multilamellar, and spherical with a smoother surface as determined by transmission electron microscopy. SCF-CO2 liposomes showed no significant differences in their particle size and PDI after more than 3 months, whereas conventional liposomes exhibited significant changes in their particle size. The initial yield (%), EE (%), and DL (%) of SCF-CO2 liposomes and conventional liposomes were 90.98 ± 2.94, 92.20 ± 1.36, 20.99 ± 0.84 and 90.72 ± 2.83, 90.24 ± 1.37, 20.47 ± 0.94, respectively, which changed after 14 weeks to 86.65 ± 0.30, 87.63 ± 0.72, 18.98 ± 0.22 and 75.04 ± 8.80, 84.59 ± 5.13, 15.94 ± 2.80, respectively. Therefore, the newly developed SCF-CO2 method could be a better alternative compared with the conventional method and may provide a promising approach for large-scale production of liposomes.

Original languageEnglish
Pages (from-to)365-377
Number of pages13
JournalInternational Journal of Nanomedicine
Volume8
DOIs
Publication statusPublished - 2013 Jan 21

Fingerprint

Supercritical fluids
Liposomes
Cyclosporine
Particle Size
Polydispersity
Particle size
Pharmaceutical Preparations
High performance liquid chromatography
Reverse-Phase Chromatography
Zeta potential
Transmission Electron Microscopy
Carbon Dioxide
Carbon dioxide
High Pressure Liquid Chromatography
Transmission electron microscopy

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Drug Discovery
  • Organic Chemistry

Cite this

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title = "Characterization and stability studies of a novel liposomal cyclosporin a prepared using the supercritical fluid method: Comparison with the modified conventional Bangham method",
abstract = "A novel method to prepare cyclosporin A encapsulated liposomes was introduced using supercritical fluid of carbon dioxide (SCF-CO2) as an antisolvent. To investigate the strength of the newly developed SCF-CO2 method compared with the modified conventional Bangham method, particle size, zeta potential, and polydispersity index (PDI) of both liposomal formulations were characterized and compared. In addition, entrapment efficiency (EE) and drug loading (DL) characteristics were analyzed by reversed-phase high-performance liquid chromatography. Significantly larger particle size and PDI were revealed from the conventional method, while EE ({\%}) and DL ({\%}) did not exhibit any significant differences. The SCF-CO2 liposomes were found to be relatively smaller, multilamellar, and spherical with a smoother surface as determined by transmission electron microscopy. SCF-CO2 liposomes showed no significant differences in their particle size and PDI after more than 3 months, whereas conventional liposomes exhibited significant changes in their particle size. The initial yield ({\%}), EE ({\%}), and DL ({\%}) of SCF-CO2 liposomes and conventional liposomes were 90.98 ± 2.94, 92.20 ± 1.36, 20.99 ± 0.84 and 90.72 ± 2.83, 90.24 ± 1.37, 20.47 ± 0.94, respectively, which changed after 14 weeks to 86.65 ± 0.30, 87.63 ± 0.72, 18.98 ± 0.22 and 75.04 ± 8.80, 84.59 ± 5.13, 15.94 ± 2.80, respectively. Therefore, the newly developed SCF-CO2 method could be a better alternative compared with the conventional method and may provide a promising approach for large-scale production of liposomes.",
author = "Karn, {Pankaj Ranjan} and Wonkyung Cho and Park, {Hee Jun} and Park, {Jeong Sook} and Hwang, {Sung Joo}",
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Characterization and stability studies of a novel liposomal cyclosporin a prepared using the supercritical fluid method : Comparison with the modified conventional Bangham method. / Karn, Pankaj Ranjan; Cho, Wonkyung; Park, Hee Jun; Park, Jeong Sook; Hwang, Sung Joo.

In: International Journal of Nanomedicine, Vol. 8, 21.01.2013, p. 365-377.

Research output: Contribution to journalArticle

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