Characterization and tissue incorporation of cross-linked human acellular dermal matrix

JuHee Lee, Hyung Goo Kim, Won Jai Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Here, we describe a novel human acellular dermal matrix (ADM) cross-linked using electron beam irradiation. Structural and biomechanical characteristics of the human ADM were assessed by infrared spectrometry and uni-axial tensile testing. Electron beam irradiation affects collagen secondary structure, which can be detected in the amide I spectral region (1660cm-1 and 1690cm-1). At doses exceeding 25kGy, cross-linking of the collagen matrix results in a denser, more stratified appearance and parallel arrangement, with significantly increased tensile strength and elastic modulus. In a micropig model, the implanted ADM elicits rapid host cell infiltration and extracellular matrix deposition; however, the delayed remodeling resulted in long-term structural integrity. Furthermore, mean densities of collagen and elastin, expression of extracellular matrix proteins, and microvessel formation within the implanted ADM increased significantly, whereas the thickness of the implanted ADM did not decrease during the course of the study. Compared with normal adjacent tissue, type I collagen mRNA levels in the ADM increased 12-fold at 3 months after implantation, and transforming growth factor-β mRNA levels increased 3.3-fold at 2 months. Matrix metalloproteinase (MMP)-1 and MMP-9 mRNA levels were also elevated. Collectively, these results demonstrate that the structural and biomechanical properties of this novel cross-linked human ADM are adequate for use as a biologic tissue substitute.

Original languageEnglish
Pages (from-to)195-205
Number of pages11
JournalBiomaterials
Volume44
DOIs
Publication statusPublished - 2015 Jan 1

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Acellular Dermis
Collagen
Tissue
Messenger RNA
Electron beams
Irradiation
Matrix Metalloproteinase 1
Elastin
Extracellular Matrix Proteins
Matrix Metalloproteinase 9
Tensile testing
Transforming Growth Factors
Structural integrity
Collagen Type I
Infiltration
Amides
Spectrometry
Tensile strength
Elastic moduli
Infrared radiation

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

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Characterization and tissue incorporation of cross-linked human acellular dermal matrix. / Lee, JuHee; Kim, Hyung Goo; Lee, Won Jai.

In: Biomaterials, Vol. 44, 01.01.2015, p. 195-205.

Research output: Contribution to journalArticle

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