Abstract
Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO 2 (RITC). We also conducted various in vivo-uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO 2 (RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO 2 (RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO 2 (RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc SCID (NOD-SCID) mice. These results demonstrate that MNPs@SiO 2 (RITC) are biocompatible and useful tools for human MSC labeling and bioimaging. From the Clinical Editor: The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated.
| Original language | English |
|---|---|
| Pages (from-to) | 263-276 |
| Number of pages | 14 |
| Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
| Volume | 6 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 Apr 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Bioengineering
- Medicine (miscellaneous)
- Molecular Medicine
- Biomedical Engineering
- Materials Science(all)
- Pharmaceutical Science
Cite this
}
Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood-derived mesenchymal stem cells. / Park, Ki Soo; Tae, Jinsung; Choi, Bongkum; Kim, Young Seok; Moon, Cheol; Kim, Sa Hyun; Lee, Han Sin; Kim, Jinhyun; Kim, Junsung; Park, Jaeberm; Lee, Jung Hee; Lee, Jongeun; Joh, Jae Won; Kim, Sungjoo.
In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 6, No. 2, 01.04.2010, p. 263-276.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood-derived mesenchymal stem cells
AU - Park, Ki Soo
AU - Tae, Jinsung
AU - Choi, Bongkum
AU - Kim, Young Seok
AU - Moon, Cheol
AU - Kim, Sa Hyun
AU - Lee, Han Sin
AU - Kim, Jinhyun
AU - Kim, Junsung
AU - Park, Jaeberm
AU - Lee, Jung Hee
AU - Lee, Jongeun
AU - Joh, Jae Won
AU - Kim, Sungjoo
PY - 2010/4/1
Y1 - 2010/4/1
N2 - Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO 2 (RITC). We also conducted various in vivo-uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO 2 (RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO 2 (RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO 2 (RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc SCID (NOD-SCID) mice. These results demonstrate that MNPs@SiO 2 (RITC) are biocompatible and useful tools for human MSC labeling and bioimaging. From the Clinical Editor: The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated.
AB - Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO 2 (RITC). We also conducted various in vivo-uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO 2 (RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO 2 (RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO 2 (RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc SCID (NOD-SCID) mice. These results demonstrate that MNPs@SiO 2 (RITC) are biocompatible and useful tools for human MSC labeling and bioimaging. From the Clinical Editor: The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=77950300683&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950300683&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2009.07.005
DO - 10.1016/j.nano.2009.07.005
M3 - Article
VL - 6
SP - 263
EP - 276
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 2
ER -