Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration

Yeonsue Jang, Yong Gon Koh, Yun Jin Choi, Sung Hwan Kim, Dong Suk Yoon, Moses Lee, jinwoo lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration. However, AT-SVF is not fully characterized in terms of functional equivalence to BMC. Therefore, in this study, we characterized AT-SVF and assessed its suitability as a one-step surgical procedure for cartilage regeneration, as an alternative to BMC. AT-SVF contained approximately sixfold less nucleated cells than BMC. However, adherent cells in AT-SVF were fourfold greater than BMC. Additionally, the colony-forming unit frequency of AT-SVF was higher than that of BMC, at 0.5 and 0.01%, respectively. The mesenchymal stem cell (MSC) population (CD45−CD31−CD90+CD105+) was 4.28% in AT-SVF and 0.42% in BMC, and the adipose-derived stromal cell (ASC) population (CD34+CD31−CD146−) was 32% in AT-SVF and 0.16% in BMC. In vitro chondrogenesis demonstrated that micromass was not formed in BMC, whereas it was clearly formed in AT-SVF. Taken together, uncultured AT-SVF could be used in one-step surgery for cartilage regeneration as a substitute for BMC.

Original languageEnglish
Pages (from-to)142-150
Number of pages9
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume51
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Cartilage
Blood Vessels
Adipose Tissue
Regeneration
Bone Marrow
Stem Cells
Mesenchymal Stromal Cells
Chondrogenesis
Stromal Cells
Bone Marrow Cells
Population
Cell Count

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Jang, Yeonsue ; Koh, Yong Gon ; Choi, Yun Jin ; Kim, Sung Hwan ; Yoon, Dong Suk ; Lee, Moses ; lee, jinwoo. / Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration. In: In Vitro Cellular and Developmental Biology - Animal. 2014 ; Vol. 51, No. 2. pp. 142-150.
@article{6fab57b386de4e30bf0b8e579e84e947,
title = "Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration",
abstract = "Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration. However, AT-SVF is not fully characterized in terms of functional equivalence to BMC. Therefore, in this study, we characterized AT-SVF and assessed its suitability as a one-step surgical procedure for cartilage regeneration, as an alternative to BMC. AT-SVF contained approximately sixfold less nucleated cells than BMC. However, adherent cells in AT-SVF were fourfold greater than BMC. Additionally, the colony-forming unit frequency of AT-SVF was higher than that of BMC, at 0.5 and 0.01{\%}, respectively. The mesenchymal stem cell (MSC) population (CD45−CD31−CD90+CD105+) was 4.28{\%} in AT-SVF and 0.42{\%} in BMC, and the adipose-derived stromal cell (ASC) population (CD34+CD31−CD146−) was 32{\%} in AT-SVF and 0.16{\%} in BMC. In vitro chondrogenesis demonstrated that micromass was not formed in BMC, whereas it was clearly formed in AT-SVF. Taken together, uncultured AT-SVF could be used in one-step surgery for cartilage regeneration as a substitute for BMC.",
author = "Yeonsue Jang and Koh, {Yong Gon} and Choi, {Yun Jin} and Kim, {Sung Hwan} and Yoon, {Dong Suk} and Moses Lee and jinwoo lee",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/s11626-014-9814-6",
language = "English",
volume = "51",
pages = "142--150",
journal = "In Vitro Cellular and Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer New York",
number = "2",

}

Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration. / Jang, Yeonsue; Koh, Yong Gon; Choi, Yun Jin; Kim, Sung Hwan; Yoon, Dong Suk; Lee, Moses; lee, jinwoo.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 51, No. 2, 01.01.2014, p. 142-150.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of adipose tissue-derived stromal vascular fraction for clinical application to cartilage regeneration

AU - Jang, Yeonsue

AU - Koh, Yong Gon

AU - Choi, Yun Jin

AU - Kim, Sung Hwan

AU - Yoon, Dong Suk

AU - Lee, Moses

AU - lee, jinwoo

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration. However, AT-SVF is not fully characterized in terms of functional equivalence to BMC. Therefore, in this study, we characterized AT-SVF and assessed its suitability as a one-step surgical procedure for cartilage regeneration, as an alternative to BMC. AT-SVF contained approximately sixfold less nucleated cells than BMC. However, adherent cells in AT-SVF were fourfold greater than BMC. Additionally, the colony-forming unit frequency of AT-SVF was higher than that of BMC, at 0.5 and 0.01%, respectively. The mesenchymal stem cell (MSC) population (CD45−CD31−CD90+CD105+) was 4.28% in AT-SVF and 0.42% in BMC, and the adipose-derived stromal cell (ASC) population (CD34+CD31−CD146−) was 32% in AT-SVF and 0.16% in BMC. In vitro chondrogenesis demonstrated that micromass was not formed in BMC, whereas it was clearly formed in AT-SVF. Taken together, uncultured AT-SVF could be used in one-step surgery for cartilage regeneration as a substitute for BMC.

AB - Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration. However, AT-SVF is not fully characterized in terms of functional equivalence to BMC. Therefore, in this study, we characterized AT-SVF and assessed its suitability as a one-step surgical procedure for cartilage regeneration, as an alternative to BMC. AT-SVF contained approximately sixfold less nucleated cells than BMC. However, adherent cells in AT-SVF were fourfold greater than BMC. Additionally, the colony-forming unit frequency of AT-SVF was higher than that of BMC, at 0.5 and 0.01%, respectively. The mesenchymal stem cell (MSC) population (CD45−CD31−CD90+CD105+) was 4.28% in AT-SVF and 0.42% in BMC, and the adipose-derived stromal cell (ASC) population (CD34+CD31−CD146−) was 32% in AT-SVF and 0.16% in BMC. In vitro chondrogenesis demonstrated that micromass was not formed in BMC, whereas it was clearly formed in AT-SVF. Taken together, uncultured AT-SVF could be used in one-step surgery for cartilage regeneration as a substitute for BMC.

UR - http://www.scopus.com/inward/record.url?scp=84925507776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925507776&partnerID=8YFLogxK

U2 - 10.1007/s11626-014-9814-6

DO - 10.1007/s11626-014-9814-6

M3 - Article

VL - 51

SP - 142

EP - 150

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

SN - 1071-2690

IS - 2

ER -