Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032

Pu Hyeon Cha, Sun Yung Park, Min Woo Moon, Bindu Subhadra, Tae Kwang Oh, Eungbin Kim, Jihyun F. Kim, Jung Kee Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.

Original languageEnglish
Pages (from-to)1061-1068
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume85
Issue number4
DOIs
Publication statusPublished - 2010 Jan

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Corynebacterium glutamicum
Gene Deletion
Adenylyl Cyclases
Acetates
Glucose
Propionates
Enzymes
Growth
Cyclic AMP
Genes
Membrane Proteins
Ethanol
Genome
glyoxylic acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Cha, Pu Hyeon ; Park, Sun Yung ; Moon, Min Woo ; Subhadra, Bindu ; Oh, Tae Kwang ; Kim, Eungbin ; Kim, Jihyun F. ; Lee, Jung Kee. / Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032. In: Applied Microbiology and Biotechnology. 2010 ; Vol. 85, No. 4. pp. 1061-1068.
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abstract = "Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.",
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Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032. / Cha, Pu Hyeon; Park, Sun Yung; Moon, Min Woo; Subhadra, Bindu; Oh, Tae Kwang; Kim, Eungbin; Kim, Jihyun F.; Lee, Jung Kee.

In: Applied Microbiology and Biotechnology, Vol. 85, No. 4, 01.2010, p. 1061-1068.

Research output: Contribution to journalArticle

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AU - Cha, Pu Hyeon

AU - Park, Sun Yung

AU - Moon, Min Woo

AU - Subhadra, Bindu

AU - Oh, Tae Kwang

AU - Kim, Eungbin

AU - Kim, Jihyun F.

AU - Lee, Jung Kee

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N2 - Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.

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