Abstract
Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.
| Original language | English |
|---|---|
| Pages (from-to) | 1061-1068 |
| Number of pages | 8 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 85 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2010 Jan |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
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Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032. / Cha, Pu Hyeon; Park, Sun Yung; Moon, Min Woo; Subhadra, Bindu; Oh, Tae Kwang; Kim, Eungbin; Kim, Jihyun F.; Lee, Jung Kee.
In: Applied Microbiology and Biotechnology, Vol. 85, No. 4, 01.2010, p. 1061-1068.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization of an adenylate cyclase gene (cyaB) deletion mutant of Corynebacterium glutamicum ATCC 13032
AU - Cha, Pu Hyeon
AU - Park, Sun Yung
AU - Moon, Min Woo
AU - Subhadra, Bindu
AU - Oh, Tae Kwang
AU - Kim, Eungbin
AU - Kim, Jihyun F.
AU - Lee, Jung Kee
PY - 2010/1
Y1 - 2010/1
N2 - Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.
AB - Genome analysis of C. glutamicum ATCC 13032 has showed one putative adenylate cyclase gene, cyaB (cg0375) which encodes membrane protein belonging to class III adenylate cyclases. To characterize the function of cyaB, a deletion mutant was constructed, and the mutant showed decreased level of intracellular cyclic AMP compared to that of wild-type. Interestingly, the cyaB mutant displayed growth defect on acetate medium, and this effect was reversed by complementation with cyaB gene. Similarly, it showed growth defect on glucose-acetate mixture minimal medium, and the utilization of glucose was retarded in the presence of acetate. The deletion mutant retained the activity of glyoxylate bypass enzymes. Additionally, the mutant could grow on ethanol but not on propionate medium. The data obtained from this study suggests that adenylate cyclase plays an essential role in the acetate metabolism of C. glutamicum, even though detailed regulatory mechanisms involving cAMP are not yet clearly defined. The observation that glyoxylate bypass enzymes are derepressed in cyaB mutant indicates the involvement of cAMP in the repression of aceB and aceA.
UR - http://www.scopus.com/inward/record.url?scp=76649124874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76649124874&partnerID=8YFLogxK
U2 - 10.1007/s00253-009-2066-9
DO - 10.1007/s00253-009-2066-9
M3 - Article
C2 - 19568747
AN - SCOPUS:76649124874
VL - 85
SP - 1061
EP - 1068
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 4
ER -