Characterization of an mDock5-specific antibody and tissue-specific expression and subcellular localization of mDock5

Eunmin Kim, Jong-Bok Yoon, Sung Joo Kim Yoon

Research output: Contribution to journalArticle

Abstract

Rho GTPase controls multiple signal-transduction pathways involving the actin cytoskeleton and the microtubule cytoskeleton in processes such as the cell cycle, morphogenesis, and cell migration. The activity of Rho GTPases, such as Rac1, Cdc42, and RhoA, is regulated by GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs), and guanine nucleotide dissociation inhibitor (GDI). The GEFs activate Rho GTPases through exchange of GDP for GTP. A group of the GEF family, CDM-GEF, containing the Dock180 Homology Region 2 (DHR2) domain is sub-divided into four groups based on amino acid sequences. One of these subgroups, DOCK-A, includes DOCK1, DOCK2, and DOCK5, and activates Rac1 Rho GTPase. Mouse Dock5 (mDock5) is structurally similar to DOCK1 and composed of 1868 amino acids containing Src homology 3 (SH3), DHR1, DHR2, and a proline-rich (PR) motif. We generated an mDock5-specific antibody, which detected denatured and nascent forms of mDock5. We determined tissue-specific expression patterns and subcellular localization of mDock5 using this antibody. This antibody provides a way to delineate the biological functions of mDock5 in vivo.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalProtein and Peptide Letters
Volume17
Issue number4
DOIs
Publication statusPublished - 2010 Mar 31

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rho GTP-Binding Proteins
Guanine Nucleotide Exchange Factors
Tissue
Antibodies
Guanine Nucleotide Dissociation Inhibitors
GTPase-Activating Proteins
Amino Acids
Signal transduction
Nucleotides
Guanosine Triphosphate
Proline
Actins
Cytoskeleton
Actin Cytoskeleton
Morphogenesis
Microtubules
Cells
Cell Movement
Amino Acid Sequence
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

Cite this

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title = "Characterization of an mDock5-specific antibody and tissue-specific expression and subcellular localization of mDock5",
abstract = "Rho GTPase controls multiple signal-transduction pathways involving the actin cytoskeleton and the microtubule cytoskeleton in processes such as the cell cycle, morphogenesis, and cell migration. The activity of Rho GTPases, such as Rac1, Cdc42, and RhoA, is regulated by GTPase-activating proteins (GAPs), guanine nucleotide exchange factors (GEFs), and guanine nucleotide dissociation inhibitor (GDI). The GEFs activate Rho GTPases through exchange of GDP for GTP. A group of the GEF family, CDM-GEF, containing the Dock180 Homology Region 2 (DHR2) domain is sub-divided into four groups based on amino acid sequences. One of these subgroups, DOCK-A, includes DOCK1, DOCK2, and DOCK5, and activates Rac1 Rho GTPase. Mouse Dock5 (mDock5) is structurally similar to DOCK1 and composed of 1868 amino acids containing Src homology 3 (SH3), DHR1, DHR2, and a proline-rich (PR) motif. We generated an mDock5-specific antibody, which detected denatured and nascent forms of mDock5. We determined tissue-specific expression patterns and subcellular localization of mDock5 using this antibody. This antibody provides a way to delineate the biological functions of mDock5 in vivo.",
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Characterization of an mDock5-specific antibody and tissue-specific expression and subcellular localization of mDock5. / Kim, Eunmin; Yoon, Jong-Bok; Yoon, Sung Joo Kim.

In: Protein and Peptide Letters, Vol. 17, No. 4, 31.03.2010, p. 485-491.

Research output: Contribution to journalArticle

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