Characterization of bla CMY-11, an AmpC-type plasmid-mediated β-lactamase gene in a Korean clinical isolate of Escherichia coli

Sang Hee Lee, Jae Young Kim, Gyu Sang Lee, Seok Ho Cheon, Young Jun An, Seok Hoon Jeong, Kye Joon Lee

Research output: Contribution to journalArticle

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Abstract

We report the description of a new plasmid-encoded AmpC-type β-lactamase gene (bla CMY-11) from Escherichia coli K983802.1 that was isolated from a patient in South Korea suffering from a urinary tract infection. Antibiotic susceptibility testing, plasmid analysis, pl determination, transconjugation and Southern blot analysis were carried out to investigate the resistance mechanism to cefoxitin. PCR, sequencing and sequence analysis were used to identify and analyse the β-lactamase gene (bla CMY-11) responsible for the cefoxitin resistance. CMY-11 and bla CMY-11 are compared with other class C β-lactamase and their genes to determine phylogenetic relationships. The cefoxitin-resistance phenotype of E. coli K983802.1 reflects the presence of a large plasmid [pYMG-2 (130 kb)]. A β-lactamase with a pl value of 8.0 from a transconjugant of E. coli K983802.1 was identified by isoelectric focusing. A 1478 bp DNA fragment from pYMG-2 containing bla CMY-11 was sequenced and an open reading frame coding for a 382 amino acid peptide (CMY-11) was found. Phylogenetic analysis clearly shows that bla CMY-11 belongs to the group of ampC-related bla genes. It is likely that bla CMY-11 evolved from bla CMY-1 via bla CMY-10.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume49
Issue number2
Publication statusPublished - 2002 Mar 4

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Cefoxitin
Plasmids
Escherichia coli
Genes
Republic of Korea
Isoelectric Focusing
Southern Blotting
Urinary Tract Infections
Open Reading Frames
Sequence Analysis
Anti-Bacterial Agents
Phenotype
Amino Acids
Polymerase Chain Reaction
Peptides
DNA

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Lee, Sang Hee ; Kim, Jae Young ; Lee, Gyu Sang ; Cheon, Seok Ho ; An, Young Jun ; Jeong, Seok Hoon ; Lee, Kye Joon. / Characterization of bla CMY-11, an AmpC-type plasmid-mediated β-lactamase gene in a Korean clinical isolate of Escherichia coli. In: Journal of Antimicrobial Chemotherapy. 2002 ; Vol. 49, No. 2. pp. 269-273.
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abstract = "We report the description of a new plasmid-encoded AmpC-type β-lactamase gene (bla CMY-11) from Escherichia coli K983802.1 that was isolated from a patient in South Korea suffering from a urinary tract infection. Antibiotic susceptibility testing, plasmid analysis, pl determination, transconjugation and Southern blot analysis were carried out to investigate the resistance mechanism to cefoxitin. PCR, sequencing and sequence analysis were used to identify and analyse the β-lactamase gene (bla CMY-11) responsible for the cefoxitin resistance. CMY-11 and bla CMY-11 are compared with other class C β-lactamase and their genes to determine phylogenetic relationships. The cefoxitin-resistance phenotype of E. coli K983802.1 reflects the presence of a large plasmid [pYMG-2 (130 kb)]. A β-lactamase with a pl value of 8.0 from a transconjugant of E. coli K983802.1 was identified by isoelectric focusing. A 1478 bp DNA fragment from pYMG-2 containing bla CMY-11 was sequenced and an open reading frame coding for a 382 amino acid peptide (CMY-11) was found. Phylogenetic analysis clearly shows that bla CMY-11 belongs to the group of ampC-related bla genes. It is likely that bla CMY-11 evolved from bla CMY-1 via bla CMY-10.",
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Characterization of bla CMY-11, an AmpC-type plasmid-mediated β-lactamase gene in a Korean clinical isolate of Escherichia coli. / Lee, Sang Hee; Kim, Jae Young; Lee, Gyu Sang; Cheon, Seok Ho; An, Young Jun; Jeong, Seok Hoon; Lee, Kye Joon.

In: Journal of Antimicrobial Chemotherapy, Vol. 49, No. 2, 04.03.2002, p. 269-273.

Research output: Contribution to journalArticle

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AU - Lee, Sang Hee

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N2 - We report the description of a new plasmid-encoded AmpC-type β-lactamase gene (bla CMY-11) from Escherichia coli K983802.1 that was isolated from a patient in South Korea suffering from a urinary tract infection. Antibiotic susceptibility testing, plasmid analysis, pl determination, transconjugation and Southern blot analysis were carried out to investigate the resistance mechanism to cefoxitin. PCR, sequencing and sequence analysis were used to identify and analyse the β-lactamase gene (bla CMY-11) responsible for the cefoxitin resistance. CMY-11 and bla CMY-11 are compared with other class C β-lactamase and their genes to determine phylogenetic relationships. The cefoxitin-resistance phenotype of E. coli K983802.1 reflects the presence of a large plasmid [pYMG-2 (130 kb)]. A β-lactamase with a pl value of 8.0 from a transconjugant of E. coli K983802.1 was identified by isoelectric focusing. A 1478 bp DNA fragment from pYMG-2 containing bla CMY-11 was sequenced and an open reading frame coding for a 382 amino acid peptide (CMY-11) was found. Phylogenetic analysis clearly shows that bla CMY-11 belongs to the group of ampC-related bla genes. It is likely that bla CMY-11 evolved from bla CMY-1 via bla CMY-10.

AB - We report the description of a new plasmid-encoded AmpC-type β-lactamase gene (bla CMY-11) from Escherichia coli K983802.1 that was isolated from a patient in South Korea suffering from a urinary tract infection. Antibiotic susceptibility testing, plasmid analysis, pl determination, transconjugation and Southern blot analysis were carried out to investigate the resistance mechanism to cefoxitin. PCR, sequencing and sequence analysis were used to identify and analyse the β-lactamase gene (bla CMY-11) responsible for the cefoxitin resistance. CMY-11 and bla CMY-11 are compared with other class C β-lactamase and their genes to determine phylogenetic relationships. The cefoxitin-resistance phenotype of E. coli K983802.1 reflects the presence of a large plasmid [pYMG-2 (130 kb)]. A β-lactamase with a pl value of 8.0 from a transconjugant of E. coli K983802.1 was identified by isoelectric focusing. A 1478 bp DNA fragment from pYMG-2 containing bla CMY-11 was sequenced and an open reading frame coding for a 382 amino acid peptide (CMY-11) was found. Phylogenetic analysis clearly shows that bla CMY-11 belongs to the group of ampC-related bla genes. It is likely that bla CMY-11 evolved from bla CMY-1 via bla CMY-10.

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