Abstract
We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER stress marker genes BiP-like protein (BLP) genes, HSP70, and Bax Inhibitor-1. Furthermore, NbBPS1 deficiency led to relocalization of bZIP28 transcription factor from the ER membrane to the nucleus, similar to the bZIP28 relocalization during tunicamycin-induced ER stress. Abnormal accumulation of vesicles and increased autophagy activity were also observed in the affected leaf cells. These results suggest that inactivation of NbBPS1 function in the ER leads to ER stress, autophagy, and PCD activation in N. benthamiana.
| Original language | English |
|---|---|
| Pages (from-to) | 185-191 |
| Number of pages | 7 |
| Journal | Molecules and cells |
| Volume | 34 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
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Characterization of cell death induced by NbBPS1 silencing in nicotiana benthamiana. / Kang, Yong Won; Jeon, Young; Pai, Hyun Sook.
In: Molecules and cells, Vol. 34, No. 2, 01.08.2012, p. 185-191.Research output: Contribution to journal › Article
TY - JOUR
T1 - Characterization of cell death induced by NbBPS1 silencing in nicotiana benthamiana
AU - Kang, Yong Won
AU - Jeon, Young
AU - Pai, Hyun Sook
PY - 2012/8/1
Y1 - 2012/8/1
N2 - We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER stress marker genes BiP-like protein (BLP) genes, HSP70, and Bax Inhibitor-1. Furthermore, NbBPS1 deficiency led to relocalization of bZIP28 transcription factor from the ER membrane to the nucleus, similar to the bZIP28 relocalization during tunicamycin-induced ER stress. Abnormal accumulation of vesicles and increased autophagy activity were also observed in the affected leaf cells. These results suggest that inactivation of NbBPS1 function in the ER leads to ER stress, autophagy, and PCD activation in N. benthamiana.
AB - We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER stress marker genes BiP-like protein (BLP) genes, HSP70, and Bax Inhibitor-1. Furthermore, NbBPS1 deficiency led to relocalization of bZIP28 transcription factor from the ER membrane to the nucleus, similar to the bZIP28 relocalization during tunicamycin-induced ER stress. Abnormal accumulation of vesicles and increased autophagy activity were also observed in the affected leaf cells. These results suggest that inactivation of NbBPS1 function in the ER leads to ER stress, autophagy, and PCD activation in N. benthamiana.
UR - http://www.scopus.com/inward/record.url?scp=84865311569&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865311569&partnerID=8YFLogxK
U2 - 10.1007/s10059-012-0096-0
DO - 10.1007/s10059-012-0096-0
M3 - Article
C2 - 22729372
AN - SCOPUS:84865311569
VL - 34
SP - 185
EP - 191
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 2
ER -