Characterization of cell death induced by NbBPS1 silencing in nicotiana benthamiana

Yong Won Kang, Young Jeon, Hyunsook Pai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER stress marker genes BiP-like protein (BLP) genes, HSP70, and Bax Inhibitor-1. Furthermore, NbBPS1 deficiency led to relocalization of bZIP28 transcription factor from the ER membrane to the nucleus, similar to the bZIP28 relocalization during tunicamycin-induced ER stress. Abnormal accumulation of vesicles and increased autophagy activity were also observed in the affected leaf cells. These results suggest that inactivation of NbBPS1 function in the ER leads to ER stress, autophagy, and PCD activation in N. benthamiana.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
JournalMolecules and cells
Volume34
Issue number2
DOIs
Publication statusPublished - 2012 Aug 1

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Endoplasmic Reticulum Stress
Tobacco
Cell Death
Endoplasmic Reticulum
Autophagy
Tunicamycin
bcl-2-Associated X Protein
Reactive Oxygen Species
Proteins
Transcription Factors
Membranes
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Characterization of cell death induced by NbBPS1 silencing in nicotiana benthamiana",
abstract = "We previously showed that silencing of NbBPS1 encoding an endoplasmic reticulum (ER)-localized protein results in pleiotrophic developmental defects and cell death in Nicotiana benthamiana [Kang et al. (2008)]. In this study, we investigated the mechanism of the cell death caused by NbBPS1 silencing. Affected leaf cells exhibited morphological markers of programmed cell death (PCD) and accumulated excessive amounts of reactive oxygen species. NbBPS1 silencing caused dramatic induction of the ER stress marker genes BiP-like protein (BLP) genes, HSP70, and Bax Inhibitor-1. Furthermore, NbBPS1 deficiency led to relocalization of bZIP28 transcription factor from the ER membrane to the nucleus, similar to the bZIP28 relocalization during tunicamycin-induced ER stress. Abnormal accumulation of vesicles and increased autophagy activity were also observed in the affected leaf cells. These results suggest that inactivation of NbBPS1 function in the ER leads to ER stress, autophagy, and PCD activation in N. benthamiana.",
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Characterization of cell death induced by NbBPS1 silencing in nicotiana benthamiana. / Kang, Yong Won; Jeon, Young; Pai, Hyunsook.

In: Molecules and cells, Vol. 34, No. 2, 01.08.2012, p. 185-191.

Research output: Contribution to journalArticle

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