Characterization of immune responses to Mycobacterium tuberculosis Rv2041c protein

Su Young Kim, A. Rum Shin, Byung Soo Lee, Hwa Jung Kim, Bo Young Jeon, Sang Nae Cho, Jeong Kyu Park, Sung Jae Shill

Research output: Contribution to journalArticle

Abstract

Tuberculosis, which is caused by Mycobacterium tuberculosis (M tb), is one of the most important infectious diseases in the world. Although many functional studies have been conducted on M tb proteins in the post-genomic era, little is known about the function of many proteins expressed specifically during latency. Previously, we reported that Rv2041c from M tb H37Rv is highly expressed under conditions of low pH and hypoxia, which represent the in vitro mimicry of latent tuberculosis. In the present study, increased expression levels of Rv2041c under hypoxia and low pH in vitro culture was confirmed by RT-PCR. Interestingly, Rv2041c showed significantly increased expression among genes of the same operon and genes belonging to the same functional group. Finally, the immune responses elicited by the recombinant (r) Rv204lc protein were investigated using ex vivo and in vivo models of M tb infection. A significantly high level of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-12p40 was detected in a dose-dependent manner by treatment of murine bone marrow-derived macrophages with rRv2041c protein. In addition, IFN-γ and TNF-α secretion increased after stimulation with purified Rv204lc protein to lymphocytes from latent and active TB mice in a modified Cornell model. In conclusion, our findings suggest that Rv204lc is a new T-cell antigen and could be a potential vaccine candidate against M tb infection by inducing a strong cellular immune response.

Original languageEnglish
Pages (from-to)183-193
Number of pages11
JournalJournal of Bacteriology and Virology
Volume39
Issue number3
DOIs
Publication statusPublished - 2009 Jan 1

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Mycobacterium tuberculosis
Mycobacterium Infections
Proteins
Interleukin-12 Subunit p40
Latent Tuberculosis
Viral Tumor Antigens
Operon
Cellular Immunity
Communicable Diseases
Interleukin-6
Tuberculosis
Vaccines
Macrophages
Lymphocytes
Cytokines
T-Lymphocytes
Gene Expression
Polymerase Chain Reaction
Genes
Hypoxia

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

Kim, Su Young ; Shin, A. Rum ; Lee, Byung Soo ; Kim, Hwa Jung ; Jeon, Bo Young ; Cho, Sang Nae ; Park, Jeong Kyu ; Shill, Sung Jae. / Characterization of immune responses to Mycobacterium tuberculosis Rv2041c protein. In: Journal of Bacteriology and Virology. 2009 ; Vol. 39, No. 3. pp. 183-193.
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Characterization of immune responses to Mycobacterium tuberculosis Rv2041c protein. / Kim, Su Young; Shin, A. Rum; Lee, Byung Soo; Kim, Hwa Jung; Jeon, Bo Young; Cho, Sang Nae; Park, Jeong Kyu; Shill, Sung Jae.

In: Journal of Bacteriology and Virology, Vol. 39, No. 3, 01.01.2009, p. 183-193.

Research output: Contribution to journalArticle

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