Characterization of IncF plasmids carrying the blaCTX-M-14 gene in clinical isolates of Escherichia coli from Korea

Juwon Kim, Il Kwon Bae, Seok Hoon Jeong, Chulhun L. Chang, Chae Hoon Lee, Kyungwon Lee

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Abstract

Objectives: The purpose of this study was to investigate the molecular epidemiology of CTX-M-14-producing Escherichia coli clinical isolates from Korea. Methods: A total of 138 non-duplicate E. coli clinical isolates showing reduced susceptibility or resistance to ceftazidime and/or cefotaxime were included in the study. Resistance genes, genetic environment, R plasmid size and replicon type, sequence type (ST) and XbaI-macrorestriction patterns were determined. Results: Among 138 isolates, 35 were found to carry the blaCTX-M-14 gene. The ISEcp1 element was identified in the upstream region of the blaCTX-M-14 gene in 32 isolates. The blaCTX-M-14 gene was located on an IncF plasmid in 21 isolates, on an IncA/C plasmid in 1 isolate, on the chromosome in 8 isolates and on both the chromosome and an IncF plasmid in 5 isolates. The most prevalent ST was ST405 (n=8), followed by ST354 (n=4), ST38 (n=3), ST69 (n=3) and the intercontinental ST, ST131 (n=3). PFGE and multilocus sequence typing experiments demonstrated no major clonal relationship among the CTX-M-14-producing isolates. Conclusions: The blaCTX-M-14 gene was probably mobilized by IncF plasmids, which can readily spread in E. coli, causing horizontal dissemination of the resistance gene in Korea.

Original languageEnglish
Article numberdkr106
Pages (from-to)1263-1268
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume66
Issue number6
DOIs
Publication statusPublished - 2011 Jun 1

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All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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