Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus

Kwang Hee Lee, Sang Uk Seo, Jae Min Song, Chung Min Lee, Hyun Ah Kim, Baik L. Seong

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A human influenza A virus X-31 (high-yielding strain) was cold-adapted for possible future use as live attenuated vaccine. Mutant influenza viruses were selected during successive serial passage in embryonated hens' eggs at progressively lower sub-optimal temperature (30, 27°C followed by 24°C). The cold-passaged mutant exhibited both temperature-sensitivity (ts) and cold-adapted (ca) phenotypes. The pathogenicity and immunogenicity of X-31 ca virus were studied in mice following intranasal inoculation. The mice did not show clinical signs even at high titer infection. Immunization of mice with X-31 ca virus elicited high titers of neutralizing antibody and provided complete protection against homologous and heterologous virus challenges. To assess the genetic stability, the X-31 ca virus was passaged at 37°C in MDCK cells or inoculated into mice. Revertant virus was not found in the lungs of any of the mice and the supernatants of the MDCK culture. We conclude that the X-31 ca candidate vaccine virus exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for live attenuated vaccine and merits further evaluation at clinical level.

Original languageEnglish
Pages (from-to)1966-1974
Number of pages9
JournalVaccine
Volume24
Issue number11
DOIs
Publication statusPublished - 2006 Mar 10

Fingerprint

Influenza Vaccines
influenza
vaccines
Viruses
viruses
live vaccines
mice
Attenuated Vaccines
immune response
Serial Passage
human influenza
mutants
Madin Darby Canine Kidney Cells
genetic stability
Influenza A virus
Orthomyxoviridae
Neutralizing Antibodies
neutralizing antibodies
Human Influenza
Eggs

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Lee, Kwang Hee ; Seo, Sang Uk ; Song, Jae Min ; Lee, Chung Min ; Kim, Hyun Ah ; Seong, Baik L. / Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus. In: Vaccine. 2006 ; Vol. 24, No. 11. pp. 1966-1974.
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Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus. / Lee, Kwang Hee; Seo, Sang Uk; Song, Jae Min; Lee, Chung Min; Kim, Hyun Ah; Seong, Baik L.

In: Vaccine, Vol. 24, No. 11, 10.03.2006, p. 1966-1974.

Research output: Contribution to journalArticle

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