Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1

Do Nyun Kim, Min Koo Seo, Hoyun Choi, Su Yeon Kim, Hee Jong Shin, A. Ran Yoon, Qian Tao, Sun Young Rha, Suk Kyeong Lee

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is a herpesvirus associated with lymphomas and carcinomas. While EBV-associated epithelial cell lines are good model systems to investigate the role of EBV in carcinoma, only a few cell lines are available as they are hard to acquire. A greater variety of naturally EBV-infected cell lines which are derived from tumour patients are needed to represent various features of EBVaGC. We characterized cell line YCCEL1, established from a Korean EBVaGC patient, to ascertain whether it can be used to study the roles of EBV in EBVaGC. The expression of EBV genes and cell surface markers was examined by in situ hybridization, RT-PCR, Western blot analysis, immunofluorescence assay and Northern blot analysis. EBV episomal status was analysed by Southern blotting and real-time PCR. This cell line expressed EBV nuclear antigen 1 (EBNA1) and latent membrane protein 2A (LMP2A), but not EBNA2, LMP2B nor LMP1. The majority of the lytic proteins were not detected in YCCEL1 cells either before or after treatment with 12-O-tetradecanoylphorbol-13-acetate. YCCEL1 cells expressed BART microRNAs (miRNAs) at high level but did not express BHRF1 miRNAs. YCCEL1 cells expressed cytokeratin, but not CD21 and CD19, suggesting CD21-independent EBV infection. The latent EBV gene and EBV miRNA expression pattern of YCCEL1 cells closely resembled that of general EBVaGC cases. Our results support the value of YCCEL1 cells as a good model system to study the role of EBV in gastric carcinogenesis.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalJournal of General Virology
Volume94
Issue numberPART3
DOIs
Publication statusPublished - 2013 Feb 25

Fingerprint

Human Herpesvirus 4
Stomach
Carcinoma
Cell Line
MicroRNAs
Epstein-Barr Virus Nuclear Antigens
Epstein-Barr Virus Infections
Herpesviridae
Tetradecanoylphorbol Acetate
Keratins
Southern Blotting
Northern Blotting
Genes
In Situ Hybridization
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Lymphoma
Membrane Proteins
Carcinogenesis
Western Blotting

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Kim, D. N., Seo, M. K., Choi, H., Kim, S. Y., Shin, H. J., Yoon, A. R., ... Lee, S. K. (2013). Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1. Journal of General Virology, 94(PART3), 497-506. https://doi.org/10.1099/vir.0.045237-0
Kim, Do Nyun ; Seo, Min Koo ; Choi, Hoyun ; Kim, Su Yeon ; Shin, Hee Jong ; Yoon, A. Ran ; Tao, Qian ; Rha, Sun Young ; Lee, Suk Kyeong. / Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1. In: Journal of General Virology. 2013 ; Vol. 94, No. PART3. pp. 497-506.
@article{52b9ab0280b5430f9afae696e17f4419,
title = "Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1",
abstract = "Epstein-Barr virus (EBV) is a herpesvirus associated with lymphomas and carcinomas. While EBV-associated epithelial cell lines are good model systems to investigate the role of EBV in carcinoma, only a few cell lines are available as they are hard to acquire. A greater variety of naturally EBV-infected cell lines which are derived from tumour patients are needed to represent various features of EBVaGC. We characterized cell line YCCEL1, established from a Korean EBVaGC patient, to ascertain whether it can be used to study the roles of EBV in EBVaGC. The expression of EBV genes and cell surface markers was examined by in situ hybridization, RT-PCR, Western blot analysis, immunofluorescence assay and Northern blot analysis. EBV episomal status was analysed by Southern blotting and real-time PCR. This cell line expressed EBV nuclear antigen 1 (EBNA1) and latent membrane protein 2A (LMP2A), but not EBNA2, LMP2B nor LMP1. The majority of the lytic proteins were not detected in YCCEL1 cells either before or after treatment with 12-O-tetradecanoylphorbol-13-acetate. YCCEL1 cells expressed BART microRNAs (miRNAs) at high level but did not express BHRF1 miRNAs. YCCEL1 cells expressed cytokeratin, but not CD21 and CD19, suggesting CD21-independent EBV infection. The latent EBV gene and EBV miRNA expression pattern of YCCEL1 cells closely resembled that of general EBVaGC cases. Our results support the value of YCCEL1 cells as a good model system to study the role of EBV in gastric carcinogenesis.",
author = "Kim, {Do Nyun} and Seo, {Min Koo} and Hoyun Choi and Kim, {Su Yeon} and Shin, {Hee Jong} and Yoon, {A. Ran} and Qian Tao and Rha, {Sun Young} and Lee, {Suk Kyeong}",
year = "2013",
month = "2",
day = "25",
doi = "10.1099/vir.0.045237-0",
language = "English",
volume = "94",
pages = "497--506",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "PART3",

}

Kim, DN, Seo, MK, Choi, H, Kim, SY, Shin, HJ, Yoon, AR, Tao, Q, Rha, SY & Lee, SK 2013, 'Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1', Journal of General Virology, vol. 94, no. PART3, pp. 497-506. https://doi.org/10.1099/vir.0.045237-0

Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1. / Kim, Do Nyun; Seo, Min Koo; Choi, Hoyun; Kim, Su Yeon; Shin, Hee Jong; Yoon, A. Ran; Tao, Qian; Rha, Sun Young; Lee, Suk Kyeong.

In: Journal of General Virology, Vol. 94, No. PART3, 25.02.2013, p. 497-506.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1

AU - Kim, Do Nyun

AU - Seo, Min Koo

AU - Choi, Hoyun

AU - Kim, Su Yeon

AU - Shin, Hee Jong

AU - Yoon, A. Ran

AU - Tao, Qian

AU - Rha, Sun Young

AU - Lee, Suk Kyeong

PY - 2013/2/25

Y1 - 2013/2/25

N2 - Epstein-Barr virus (EBV) is a herpesvirus associated with lymphomas and carcinomas. While EBV-associated epithelial cell lines are good model systems to investigate the role of EBV in carcinoma, only a few cell lines are available as they are hard to acquire. A greater variety of naturally EBV-infected cell lines which are derived from tumour patients are needed to represent various features of EBVaGC. We characterized cell line YCCEL1, established from a Korean EBVaGC patient, to ascertain whether it can be used to study the roles of EBV in EBVaGC. The expression of EBV genes and cell surface markers was examined by in situ hybridization, RT-PCR, Western blot analysis, immunofluorescence assay and Northern blot analysis. EBV episomal status was analysed by Southern blotting and real-time PCR. This cell line expressed EBV nuclear antigen 1 (EBNA1) and latent membrane protein 2A (LMP2A), but not EBNA2, LMP2B nor LMP1. The majority of the lytic proteins were not detected in YCCEL1 cells either before or after treatment with 12-O-tetradecanoylphorbol-13-acetate. YCCEL1 cells expressed BART microRNAs (miRNAs) at high level but did not express BHRF1 miRNAs. YCCEL1 cells expressed cytokeratin, but not CD21 and CD19, suggesting CD21-independent EBV infection. The latent EBV gene and EBV miRNA expression pattern of YCCEL1 cells closely resembled that of general EBVaGC cases. Our results support the value of YCCEL1 cells as a good model system to study the role of EBV in gastric carcinogenesis.

AB - Epstein-Barr virus (EBV) is a herpesvirus associated with lymphomas and carcinomas. While EBV-associated epithelial cell lines are good model systems to investigate the role of EBV in carcinoma, only a few cell lines are available as they are hard to acquire. A greater variety of naturally EBV-infected cell lines which are derived from tumour patients are needed to represent various features of EBVaGC. We characterized cell line YCCEL1, established from a Korean EBVaGC patient, to ascertain whether it can be used to study the roles of EBV in EBVaGC. The expression of EBV genes and cell surface markers was examined by in situ hybridization, RT-PCR, Western blot analysis, immunofluorescence assay and Northern blot analysis. EBV episomal status was analysed by Southern blotting and real-time PCR. This cell line expressed EBV nuclear antigen 1 (EBNA1) and latent membrane protein 2A (LMP2A), but not EBNA2, LMP2B nor LMP1. The majority of the lytic proteins were not detected in YCCEL1 cells either before or after treatment with 12-O-tetradecanoylphorbol-13-acetate. YCCEL1 cells expressed BART microRNAs (miRNAs) at high level but did not express BHRF1 miRNAs. YCCEL1 cells expressed cytokeratin, but not CD21 and CD19, suggesting CD21-independent EBV infection. The latent EBV gene and EBV miRNA expression pattern of YCCEL1 cells closely resembled that of general EBVaGC cases. Our results support the value of YCCEL1 cells as a good model system to study the role of EBV in gastric carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84874067404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874067404&partnerID=8YFLogxK

U2 - 10.1099/vir.0.045237-0

DO - 10.1099/vir.0.045237-0

M3 - Article

C2 - 23175241

AN - SCOPUS:84874067404

VL - 94

SP - 497

EP - 506

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - PART3

ER -