Characterization of plasma gelsolin as a substrate for matrix metalloproteinases

Sung Min Park, In Kwan Hwang, Se Yeon Kim, Seo Jin Lee, Kang Sik Park, Seung Taek Lee

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We previously showed that plasma gelsolin, a major component of the extracellular actin scavenging system, is an matrix metalloproteinase (MMP)-14 substrate. Here we confirmed that plasma gelsolin is cleaved by MMP-14 at the plasma level, and found that it was most efficiently digested by MMP-3 followed by MMP-2, MMP-1, MMP-14, and MMP-9, in that order. Plasma gelsolin (90 kDa) was cut into several fragments of 43-48 kDa by MMP-3. The MMP-3 cleavage sites in plasma gelsolin were determined by labeling the C termini generated by in-gel digestion with 50% H218O combined with peptide mass mapping, and sequencing of the N-terminal amino acids. Plasma gelsolin was cleaved at Asn416-Val417, Ser51-Met 52, and Ala435-Gln436. Proteolytic cleavage by MMP-3 resulted in considerable loss of its actin filament-depolymerizing activity. This suggests that MMPs weaken the extracellular actin-scavenging system by cleaving plasma gelsolin and may, therefore, be involved in pathological conditions induced by extracellular actin, such as endothelial injury, respiratory distress syndrome, hepatic necrosis, and septic shock.

Original languageEnglish
Pages (from-to)1192-1199
Number of pages8
JournalProteomics
Volume6
Issue number4
DOIs
Publication statusPublished - 2006 Feb 1

Fingerprint

Gelsolin
Matrix Metalloproteinases
Matrix Metalloproteinase 3
Plasmas
Matrix Metalloproteinase 14
Substrates
Actins
Scavenging
Matrix Metalloproteinase 1
Peptide Mapping
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Septic Shock
Actin Cytoskeleton
Labeling
Digestion
Necrosis
Gels
Amino Acids
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Park, Sung Min ; Hwang, In Kwan ; Kim, Se Yeon ; Lee, Seo Jin ; Park, Kang Sik ; Lee, Seung Taek. / Characterization of plasma gelsolin as a substrate for matrix metalloproteinases. In: Proteomics. 2006 ; Vol. 6, No. 4. pp. 1192-1199.
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Characterization of plasma gelsolin as a substrate for matrix metalloproteinases. / Park, Sung Min; Hwang, In Kwan; Kim, Se Yeon; Lee, Seo Jin; Park, Kang Sik; Lee, Seung Taek.

In: Proteomics, Vol. 6, No. 4, 01.02.2006, p. 1192-1199.

Research output: Contribution to journalArticle

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