Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking

Si Nae Park, Jongchul Park, Hea Ok Kim, Min Jung Song, Hwal Suh

Research output: Contribution to journalArticle

404 Citations (Scopus)

Abstract

In order to develop a scaffolding material for tissue regeneration, porous matrices containing collagen and hyaluronic acid were fabricated by freeze drying at -20°C, -70°C or -196°C. The fabricated porous membranes were cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in a range of 1-100mM concentrations for enhancing mechanical stability of the composite matrix. Scanning electron microscope (SEM) views of the matrices demonstrated that the matrices obtained before cross-linking process had interconnected pores with mean diameters of 40, 90 or 230 μm and porosity of 58-66% according to the freezing temperature, and also the porous structures after cross-linking process were retained. The swelling test and IR spectroscopic measurement of different cross-linked membranes were carried out as a measure of the extent of cross-linking. The swelling behavior of cross-linked membranes showed no significant differences as cross-linking degree increased. FT-IR spectra showed the increase of the intensity of the absorbencies at amide bonds (1655, 1546, 1458cm-1) compared to that of CH bond (2930 cm-1). In enzymatic degradation test, EDC treated membranes showed significant enhancement of the resistance to collagenase activity in comparison with 0.625% glutaraldehyde treated membranes. In cytotoxicity test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no significant toxicity.

Original languageEnglish
Pages (from-to)1205-1212
Number of pages8
JournalBiomaterials
Volume23
Issue number4
DOIs
Publication statusPublished - 2002 Feb 15

Fingerprint

Ethyldimethylaminopropyl Carbodiimide
Hyaluronic acid
Hyaluronic Acid
Scaffolds (biology)
Collagen
Scaffolds
Membranes
Swelling
Tissue Scaffolds
Tissue regeneration
Freeze Drying
Mechanical stability
Porosity
Glutaral
Collagenases
Cytotoxicity
Amides
Freezing
Toxicity
Regeneration

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

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title = "Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking",
abstract = "In order to develop a scaffolding material for tissue regeneration, porous matrices containing collagen and hyaluronic acid were fabricated by freeze drying at -20°C, -70°C or -196°C. The fabricated porous membranes were cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) in a range of 1-100mM concentrations for enhancing mechanical stability of the composite matrix. Scanning electron microscope (SEM) views of the matrices demonstrated that the matrices obtained before cross-linking process had interconnected pores with mean diameters of 40, 90 or 230 μm and porosity of 58-66{\%} according to the freezing temperature, and also the porous structures after cross-linking process were retained. The swelling test and IR spectroscopic measurement of different cross-linked membranes were carried out as a measure of the extent of cross-linking. The swelling behavior of cross-linked membranes showed no significant differences as cross-linking degree increased. FT-IR spectra showed the increase of the intensity of the absorbencies at amide bonds (1655, 1546, 1458cm-1) compared to that of CH bond (2930 cm-1). In enzymatic degradation test, EDC treated membranes showed significant enhancement of the resistance to collagenase activity in comparison with 0.625{\%} glutaraldehyde treated membranes. In cytotoxicity test using L929 fibroblastic cells, the EDC-cross-linked membranes demonstrated no significant toxicity.",
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Characterization of porous collagen/hyaluronic acid scaffold modified by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide cross-linking. / Park, Si Nae; Park, Jongchul; Kim, Hea Ok; Song, Min Jung; Suh, Hwal.

In: Biomaterials, Vol. 23, No. 4, 15.02.2002, p. 1205-1212.

Research output: Contribution to journalArticle

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AU - Park, Si Nae

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AU - Suh, Hwal

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