Characterization of single stranded telomeric DNA-binding proteins in cultured soybean (Glycine max) cells

Chian Kwon, Kisang Kwon, In Kwon Chung, Soon Young Kim, Myeon Haeng Cho, Bin Goo Kang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have identified and characterized a protein factor in soybean (Glycine max) nuclear extracts that binds to plant single stranded telomeric DNA repeats. A single DNA-protein complex was detected in gel retardation assays using synthetic telomeres and nuclear extracts. The protein forming this complex was designated soybean (Glycine max) single stranded telomeric DNA-binding protein (Gm-STBP). Gm-STBP binds to single stranded telomeric DNA containing more than two repeats. It does not bind to Tetrahymena, human or mutated plant telomere sequences, and its binding activity is not affected by RNase treatment. Gm-STBP activity gradually decreased after suspension cultures entered stationary phase. A slower migrating band was formed with extracts of earlier and later phases of soybean suspension cultures. Our findings suggest that binding of Gm-STBP to plant single stranded telomeric DNA may play a role in the proper functioning of telomeres during development.

Original languageEnglish
Pages (from-to)503-508
Number of pages6
JournalMolecules and Cells
Volume17
Issue number3
Publication statusPublished - 2004 Jun 30

Fingerprint

DNA-Binding Proteins
Soybeans
Single-Stranded DNA
Telomere
Plant DNA
Suspensions
Tetrahymena
Proteins
Electrophoretic Mobility Shift Assay
Ribonucleases
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Kwon, Chian ; Kwon, Kisang ; Chung, In Kwon ; Kim, Soon Young ; Cho, Myeon Haeng ; Kang, Bin Goo. / Characterization of single stranded telomeric DNA-binding proteins in cultured soybean (Glycine max) cells. In: Molecules and Cells. 2004 ; Vol. 17, No. 3. pp. 503-508.
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Characterization of single stranded telomeric DNA-binding proteins in cultured soybean (Glycine max) cells. / Kwon, Chian; Kwon, Kisang; Chung, In Kwon; Kim, Soon Young; Cho, Myeon Haeng; Kang, Bin Goo.

In: Molecules and Cells, Vol. 17, No. 3, 30.06.2004, p. 503-508.

Research output: Contribution to journalArticle

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