Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG

Sang Kyu Park, Hyoung Song Lee, Seung-Taek Lee

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A 220-bp fragment of PTK7 cDNA was previously cloned from normal human melanocyte RNAs by means of the reverse transcription-polymerase chain reaction. We now report the cloning of the human full-length PTK7 cDNA and its characterization. The 1,070-amino acid PTK7 polypeptide deduced from the cDNA sequence constitutes receptor protein tyrosine kinase (RPTK), but has several unusual residues in some of the highly conserved tyrosine kinase motifs. PTK7 mRNA was expressed at the highest level in a human erythroleukemia cell line among tested samples, and at relatively high levels in liver, lung, pancreas, kidney, placenta, and melanocytes. Human PTK7 is 72% identical to chick KLG, suggesting that PTK7 is homologous or possibly orthologous to chick KLG, and that these represent a new subfamily of RPTKs.

Original languageEnglish
Pages (from-to)235-239
Number of pages5
JournalJournal of Biochemistry
Volume119
Issue number2
DOIs
Publication statusPublished - 1996 Jan 1

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Receptor Protein-Tyrosine Kinases
Complementary DNA
Melanocytes
Molecules
Leukemia, Erythroblastic, Acute
Cloning
Polymerase chain reaction
Transcription
Liver
Protein-Tyrosine Kinases
Placenta
Reverse Transcription
Organism Cloning
Pancreas
Cells
RNA
Kidney
Amino Acids
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

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abstract = "A 220-bp fragment of PTK7 cDNA was previously cloned from normal human melanocyte RNAs by means of the reverse transcription-polymerase chain reaction. We now report the cloning of the human full-length PTK7 cDNA and its characterization. The 1,070-amino acid PTK7 polypeptide deduced from the cDNA sequence constitutes receptor protein tyrosine kinase (RPTK), but has several unusual residues in some of the highly conserved tyrosine kinase motifs. PTK7 mRNA was expressed at the highest level in a human erythroleukemia cell line among tested samples, and at relatively high levels in liver, lung, pancreas, kidney, placenta, and melanocytes. Human PTK7 is 72{\%} identical to chick KLG, suggesting that PTK7 is homologous or possibly orthologous to chick KLG, and that these represent a new subfamily of RPTKs.",
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Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG. / Park, Sang Kyu; Lee, Hyoung Song; Lee, Seung-Taek.

In: Journal of Biochemistry, Vol. 119, No. 2, 01.01.1996, p. 235-239.

Research output: Contribution to journalArticle

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