Characterization of the Stiffness of Multiple Particles Trapped by Dielectrophoretic Tweezers in a Microfluidic Device

Myeonggu Son, Seungyeop Choi, Kwan Hwi Ko, Min Hyung Kim, Sei Young Lee, Jaehong Key, Young Ro Yoon, In Soo Park, Sang Woo Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Characterization of the stiffness of multiple particles trapped by tweezers-based force spectroscopy is a key step in building simple, high-throughput, and robust systems that can investigate the molecular interactions in a biological process, but the technology to characterize it in a given environment simultaneously is still lacking. We first characterized the stiffness of multiple particles trapped by dielectrophoretic (DEP) tweezers inside a microfluidic device. In this characterization, we developed a method to measure the thermal fluctuations of the trapped multiple particles with DEP tweezers by varying the heights of the particles in the given environment at the same time. Using the data measured in this controlled environment, we extracted the stiffness of the trapped particles and calculated their force. This study not only provides a simple and high-throughput method to measure the trap stiffness of multiple particles inside a microfluidic device using DEP tweezers but also inspires the application of the trapped multiple particles to investigate the dynamics in molecular interactions.

Original languageEnglish
Pages (from-to)922-927
Number of pages6
JournalLangmuir
Volume32
Issue number3
DOIs
Publication statusPublished - 2016 Jan 26

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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    Son, M., Choi, S., Ko, K. H., Kim, M. H., Lee, S. Y., Key, J., Yoon, Y. R., Park, I. S., & Lee, S. W. (2016). Characterization of the Stiffness of Multiple Particles Trapped by Dielectrophoretic Tweezers in a Microfluidic Device. Langmuir, 32(3), 922-927. https://doi.org/10.1021/acs.langmuir.5b03677