Characterization of two glyceraldehyde 3-phosphate dehydrogenase genes in Giardia lamblia

Hye Won Yang, Taisoon Yong, Jong Ho Lee, Kyung Il Im, Soon Jung Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We investigated two putative gap genes encoding glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in Giardia lamblia. While the expression of the gap2 gene was induced during encystation, gap1 was constitutively expressed. The induction of GAP2 protein during encystation was confirmed using GAP2-specific polyclonal antibodies. Complementation tests using a gap-deficient Escherichia coli showed that only the cell containing the gap1 gene, but not that with the gap2 gene, was able to restore GAPDH activity and grow in the presence of glucose as a sole carbon source. Levels of GAPDH activity were found to be constant in G. lamblia extracts at various times of encystation. These results suggest that a GAP1 protein functions as a major glycolytic enzyme, and that gap2 does not seem to encode a protein with GAPDH activity.

Original languageEnglish
Pages (from-to)646-650
Number of pages5
JournalParasitology Research
Volume88
Issue number7
DOIs
Publication statusPublished - 2002 Jul 3

Fingerprint

Giardia lamblia
Glyceraldehyde-3-Phosphate Dehydrogenases
glyceraldehyde-3-phosphate dehydrogenase
encystment
Genes
genes
Genetic Complementation Test
Proteins
proteins
polyclonal antibodies
Carbon
Escherichia coli
Gene Expression
Glucose
gene expression
glucose
Antibodies
carbon
extracts
Enzymes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Yang, Hye Won ; Yong, Taisoon ; Lee, Jong Ho ; Im, Kyung Il ; Park, Soon Jung. / Characterization of two glyceraldehyde 3-phosphate dehydrogenase genes in Giardia lamblia. In: Parasitology Research. 2002 ; Vol. 88, No. 7. pp. 646-650.
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Characterization of two glyceraldehyde 3-phosphate dehydrogenase genes in Giardia lamblia. / Yang, Hye Won; Yong, Taisoon; Lee, Jong Ho; Im, Kyung Il; Park, Soon Jung.

In: Parasitology Research, Vol. 88, No. 7, 03.07.2002, p. 646-650.

Research output: Contribution to journalArticle

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