Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films

Bramaramba Gnapareddy, Sreekantha Reddy Dugasani, Taewoo Ha, Bjorn Paulson, Taehyun Hwang, Taesung Kim, Jae Hoon Kim, Kyunghwan Oh, Sung Ha Park

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Double-stranded salmon DNA (SDNA) was doped with doxorubicin hydrochloride drug molecules (DOX) to determine the binding between DOX and SDNA, and DOX optimum doping concentration in SDNA. SDNA thin films were prepared with various concentrations of DOX by drop-casting on oxygen plasma treated glass and quartz substrates. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the binding sites for DOX in SDNA, and electrical and photoluminescence (PL) analyses were used to determine the optimum doping concentration of DOX. The FTIR spectra showed that up to a concentration of μM of DOX, there was a tendency for binding with a periodic orientation via intercalation between nucleosides. The current and PL intensity increased as the DOX concentration increased up to μM, and then as the concentration of DOX further increased, we observed a decrease in current as well as PL quenching. Finally, the optical band gap and second band onset of the transmittance spectra were analyzed to further verify the DOX binding and optimum doping concentration into SDNA thin films as a function of the DOX concentration.

Original languageEnglish
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Jul 31

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Salmon
Doxorubicin
DNA
Pharmaceutical Preparations
Quartz
Fourier Analysis
Fourier Transform Infrared Spectroscopy
Nucleosides
Glass
Binding Sites
Oxygen

All Science Journal Classification (ASJC) codes

  • General

Cite this

Gnapareddy, B., Reddy Dugasani, S., Ha, T., Paulson, B., Hwang, T., Kim, T., ... Park, S. H. (2015). Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films. Scientific reports, 5. https://doi.org/10.1038/srep12722
Gnapareddy, Bramaramba ; Reddy Dugasani, Sreekantha ; Ha, Taewoo ; Paulson, Bjorn ; Hwang, Taehyun ; Kim, Taesung ; Hoon Kim, Jae ; Oh, Kyunghwan ; Park, Sung Ha. / Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films. In: Scientific reports. 2015 ; Vol. 5.
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Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films. / Gnapareddy, Bramaramba; Reddy Dugasani, Sreekantha; Ha, Taewoo; Paulson, Bjorn; Hwang, Taehyun; Kim, Taesung; Hoon Kim, Jae; Oh, Kyunghwan; Park, Sung Ha.

In: Scientific reports, Vol. 5, 31.07.2015.

Research output: Contribution to journalArticle

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AU - Reddy Dugasani, Sreekantha

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AU - Hwang, Taehyun

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AU - Park, Sung Ha

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