Chemical constituents isolated from Polygala japonica leaves and their inhibitory effect on nitric oxide production in vitro

Seung Hyun Kim, Sin Duk Jang, Ki Yong Lee, Sang Hyun Sung, Young Choong Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A methanolic extract of dried leaves of Polygala japonica Houtt (Polygalaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Five anthraquinones chrysophanol (1), emodin (2), aloe-emodin (3), emodin 8-O-β-D-glucopyranoside (4) and trihydroxy anthraquinone (5), and four flavonoids kaempferol (6), chrysoeriol (7), kaempferol 3-gentiobioside (8) and isorhamnetin (9) were isolated from the methanolic extract using bioactivity-guided fractionation. Among them, compounds 1-4, 6 and 7 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia at the concentrations ranging from 1.0 to 100.0 μM.

Original languageEnglish
Pages (from-to)230-233
Number of pages4
JournalJournal of Enzyme Inhibition and Medicinal Chemistry
Volume24
Issue number1
DOIs
Publication statusPublished - 2009 Feb 3

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Polygala
Emodin
Anthraquinones
Microglia
Polygalaceae
Lipopolysaccharides
Nitric Oxide
Flavonoids
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

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abstract = "A methanolic extract of dried leaves of Polygala japonica Houtt (Polygalaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Five anthraquinones chrysophanol (1), emodin (2), aloe-emodin (3), emodin 8-O-β-D-glucopyranoside (4) and trihydroxy anthraquinone (5), and four flavonoids kaempferol (6), chrysoeriol (7), kaempferol 3-gentiobioside (8) and isorhamnetin (9) were isolated from the methanolic extract using bioactivity-guided fractionation. Among them, compounds 1-4, 6 and 7 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia at the concentrations ranging from 1.0 to 100.0 μM.",
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Chemical constituents isolated from Polygala japonica leaves and their inhibitory effect on nitric oxide production in vitro. / Kim, Seung Hyun; Jang, Sin Duk; Lee, Ki Yong; Sung, Sang Hyun; Kim, Young Choong.

In: Journal of Enzyme Inhibition and Medicinal Chemistry, Vol. 24, No. 1, 03.02.2009, p. 230-233.

Research output: Contribution to journalArticle

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