Chemical Controllable Gene Drive in Drosophila

Dongwoo Chae, Junwon Lee, Nayoung Lee, Kyungsoo Park, Seok Jun Moon, Hyongbum H. Kim

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Gene drive systems that propagate transgenes via super-Mendelian inheritance can potentially control insect-borne diseases and agricultural pests. However, concerns have been raised regarding unforeseen ecological consequences, and methods that prevent undesirable gene drive effects have been proposed. Here, we report a chemical-induced control of gene drive. We prepared a CRISPR-based gene drive system that can be removed by a site-specific recombinase, Rippase, the expression of which is induced by the chemical RU486 in fruit flies. Exposure of fruit flies to RU486 resulted in 7-12% removal of gene drive elements at each generation, leading to a significant reduction in gene drive-fly propagation. Mathematical modeling and simulation suggest that our system offers several advantages over a previously reported gene drive control system. Our chemical control system can provide a proof-of-principle for the reversible control of gene drive effects depending on ecological status and human needs.

Original languageEnglish
Pages (from-to)2362-2377
Number of pages16
JournalACS Synthetic Biology
Volume9
Issue number9
DOIs
Publication statusPublished - 2020 Sep 18

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) Grant [NRF-2016R1A5A2008630 to SJM; NRF-2017R1A2B3004198 to HK; NRF-2017M3A9B4062403 to H.K.]; and the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea [HI17C0676 to HK] funded by the Korean Government (MSIP); Yonsei University New Faculty Research Seed Money Grant of 2015 to HK; and a faculty research grant of Yonsei University College of Medicine for 2015 [6–2015–0086 to HK].

Publisher Copyright:
Copyright © 2020 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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