Chemical-Driven Outflow of Dissociated Amyloid Burden from Brain to Blood

Donghee Lee, Hyunjin Vincent Kim, Hye Yun Kim, Young Soo Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Amyloid-β (Aβ) deposition in the brain is a primary biomarker of Alzheimer's disease (AD) and Aβ measurement for AD diagnosis mostly depends on brain imaging and cerebrospinal fluid analyses. Blood Aβ can become a reliable surrogate biomarker if issues of low concentration for conventional laboratory instruments and uncertain correlation with brain Aβ are solved. Here, brain-to-blood efflux of Aβ is stimulated in AD transgenic mice by orally administrating a chemical that dissociates amyloid plaques and observing the subsequent increase of blood Aβ concentration. 5XFAD transgenic and wild-type mice of varying ages and genders are prepared, and blood samples of each mouse are collected six times for 12 weeks; three weeks of no treatment and additional nine weeks of daily oral administration, ad libitum, of Aβ plaque-dissociating chemical agent. By the dissociation of Aβ aggregates, the altered levels of plasma Aβ distinguish between transgenic and wild-type mice, displaying potential as an amyloid burden marker of AD brains.

Original languageEnglish
Article number2104542
JournalAdvanced Science
Volume9
Issue number12
DOIs
Publication statusPublished - 2022 Apr 25

Bibliographical note

Funding Information:
This research was supported by the Korea Health Technology R&D Project (Grant Number: HU21C0161, Y.K.) through the Korea Health Industry Development Institute (KHIDI) and Korea Dementia Research Center (KDRC), and Mid‐Career Researcher Program (Grant Number: NRF‐2021R1A2C2093916, YK; NRF‐2021R1A2C1013247, H.Y.K.), Basic Science Research Program (Grant Number: NRF‐2018R1A6A1A03023718, Y.K. and H.Y.K.), and Original Technology Research Program for Brain Science Program (Grant Number: NRF‐2018M3C7A1021858, Y.K.) through the National Research Foundation of Korea (NRF), funded by the Ministry of Health & Welfare and Ministry of Science and ICT, Republic of Korea. This research was also supported by the Post Doc. Researcher Supporting Program of 2020 through the Yonsei University Research Fund (Project Number: 2020‐12‐0028, H.Y.K.), Amyloid Solution (Y.K.), POSCO Science Fellowship of POSCO TJ Park Foundation (Y.K.), and Open Research Programs of Korea Institute of Science and Technology (Y.K.). The authors appreciate HeeYang Lee for proofreading.

Publisher Copyright:
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Chemical Engineering(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Engineering(all)
  • Physics and Astronomy(all)

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