Chemical microsensor arrays using biorecognition molecules and cells in micropatterned hydrogels

Michael Pishko, Won-Gun Koh, Vamsi Yadavalli

Research output: Contribution to conferencePaper

Abstract

In an effort to develop chemical sensor arrays to detect gaseous analytes, we present a new approach for the microfabrication of biosensor arrays based upon poly(ethylene glycol) diacrylate (PEG-DA) hydrogels patterned photolithographically on inorganic and plastic substrates. Individual microstructures of dimensions 5 to 600 micrometers with aspect ratios as high as 1.4 were created. In the former case as many as 400 elements were generated in a 1 mm square pattern. The resultant hydrogel patterns were hydrated for as long as three weeks without considerable delamination or deformation of the elements. The chemistry of these hydrogels allowed us to immobilized biomolecules with little loss of activity and permitted the control of mass transfer into the gels by changing the mesh size of the polymer network. Fluorophore labeled biorecognition molecules were encapsulated in these microstructures and detection of various analytes achieved by measuring, using a CCD camera, changes in fluorescence that occurred when analytes partition into the gels and bind to the recognition molecules. These microstructures were also used to encapsulate living cells and were integrated with microfluidic devices to create cell-based sensing devices.

Original languageEnglish
DOIs
Publication statusPublished - 2002 Dec 1
Event32nd International Conference on Environmental Systems - San Antonio, TX, United States
Duration: 2002 Jul 152002 Jul 18

Other

Other32nd International Conference on Environmental Systems
CountryUnited States
CitySan Antonio, TX
Period02/7/1502/7/18

Fingerprint

Microsensors
Hydrogels
Microstructure
Molecules
Gels
Fluorophores
Microfabrication
Sensor arrays
Biomolecules
CCD cameras
Chemical sensors
Microfluidics
Delamination
Biosensors
Polyethylene glycols
Aspect ratio
Mass transfer
Fluorescence
Cells
Plastics

All Science Journal Classification (ASJC) codes

  • Automotive Engineering
  • Safety, Risk, Reliability and Quality
  • Pollution
  • Industrial and Manufacturing Engineering

Cite this

Pishko, M., Koh, W-G., & Yadavalli, V. (2002). Chemical microsensor arrays using biorecognition molecules and cells in micropatterned hydrogels. Paper presented at 32nd International Conference on Environmental Systems, San Antonio, TX, United States. https://doi.org/10.4271/2002-01-2455
Pishko, Michael ; Koh, Won-Gun ; Yadavalli, Vamsi. / Chemical microsensor arrays using biorecognition molecules and cells in micropatterned hydrogels. Paper presented at 32nd International Conference on Environmental Systems, San Antonio, TX, United States.
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Pishko, M, Koh, W-G & Yadavalli, V 2002, 'Chemical microsensor arrays using biorecognition molecules and cells in micropatterned hydrogels' Paper presented at 32nd International Conference on Environmental Systems, San Antonio, TX, United States, 02/7/15 - 02/7/18, . https://doi.org/10.4271/2002-01-2455

Chemical microsensor arrays using biorecognition molecules and cells in micropatterned hydrogels. / Pishko, Michael; Koh, Won-Gun; Yadavalli, Vamsi.

2002. Paper presented at 32nd International Conference on Environmental Systems, San Antonio, TX, United States.

Research output: Contribution to conferencePaper

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Pishko M, Koh W-G, Yadavalli V. Chemical microsensor arrays using biorecognition molecules and cells in micropatterned hydrogels. 2002. Paper presented at 32nd International Conference on Environmental Systems, San Antonio, TX, United States. https://doi.org/10.4271/2002-01-2455