Chemical modification of chicken egg white proteins with polyethyleneoxide-maleic anhydride copolymer and their partitioning in aqueous two-phase systems

Yoshitsune Shin-Ya, Jin Won Park, Kyung Jin Hong, Koichi Fujiwara, Toshio Kajiuchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aqueous two-phase partitioning is one of the excellent bioseparation systems for proteins and other biomolecules in water-rich solutions containing polymers or salts. To improve the partition properties of model proteins, chicken egg white (CEW) proteins were chemically modified with polyethyleneoxide-maleic anhydride (PEOMA) copolymer. The modified proteins were partitioned in polyethyleneoxide-maleic acid/potassium phosphate aqueous two-phase systems. Firstly, the contribution of the chemical modification of proteins to the partition coefficient was investigated by using modified proteins with different degrees of modification (DM). The partition coefficients of modified proteins increased with increasing DM. The results suggested that the surface of the modified proteins had a greater hydrophobic property, similar to the PEOMA copolymer. Secondly, the effect of the pH on the partitioning of the modified proteins was studied. The partitioning of the modified proteins was found to be more dependent on pH in the alkaline systems. The increasing partition coefficient with pH at alkaline pH can be explained by the electrostatic repulsion between the phosphate anions and the maleic groups attached to the proteins. These results suggested that chemical modification of proteins with PEOMA could be a useful tool for separation and purification of crude protein mixtures.

Original languageEnglish
Pages (from-to)338-344
Number of pages7
JournalJournal of Chemical Technology and Biotechnology
Volume80
Issue number3
DOIs
Publication statusPublished - 2005 Mar 1

Fingerprint

Maleic Anhydrides
Egg Proteins
Chemical modification
Maleic anhydride
Chickens
partitioning
Copolymers
egg
Proteins
protein
partition coefficient
chemical
Phosphates
phosphate
Static Electricity
Biomolecules
Anions
Polymers
Membrane Proteins
Purification

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

@article{61c577a0cfa343279ee697c58eb43b57,
title = "Chemical modification of chicken egg white proteins with polyethyleneoxide-maleic anhydride copolymer and their partitioning in aqueous two-phase systems",
abstract = "Aqueous two-phase partitioning is one of the excellent bioseparation systems for proteins and other biomolecules in water-rich solutions containing polymers or salts. To improve the partition properties of model proteins, chicken egg white (CEW) proteins were chemically modified with polyethyleneoxide-maleic anhydride (PEOMA) copolymer. The modified proteins were partitioned in polyethyleneoxide-maleic acid/potassium phosphate aqueous two-phase systems. Firstly, the contribution of the chemical modification of proteins to the partition coefficient was investigated by using modified proteins with different degrees of modification (DM). The partition coefficients of modified proteins increased with increasing DM. The results suggested that the surface of the modified proteins had a greater hydrophobic property, similar to the PEOMA copolymer. Secondly, the effect of the pH on the partitioning of the modified proteins was studied. The partitioning of the modified proteins was found to be more dependent on pH in the alkaline systems. The increasing partition coefficient with pH at alkaline pH can be explained by the electrostatic repulsion between the phosphate anions and the maleic groups attached to the proteins. These results suggested that chemical modification of proteins with PEOMA could be a useful tool for separation and purification of crude protein mixtures.",
author = "Yoshitsune Shin-Ya and Park, {Jin Won} and Hong, {Kyung Jin} and Koichi Fujiwara and Toshio Kajiuchi",
year = "2005",
month = "3",
day = "1",
doi = "10.1002/jctb.1210",
language = "English",
volume = "80",
pages = "338--344",
journal = "Journal of Chemical Technology and Biotechnology",
issn = "0268-2575",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

Chemical modification of chicken egg white proteins with polyethyleneoxide-maleic anhydride copolymer and their partitioning in aqueous two-phase systems. / Shin-Ya, Yoshitsune; Park, Jin Won; Hong, Kyung Jin; Fujiwara, Koichi; Kajiuchi, Toshio.

In: Journal of Chemical Technology and Biotechnology, Vol. 80, No. 3, 01.03.2005, p. 338-344.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemical modification of chicken egg white proteins with polyethyleneoxide-maleic anhydride copolymer and their partitioning in aqueous two-phase systems

AU - Shin-Ya, Yoshitsune

AU - Park, Jin Won

AU - Hong, Kyung Jin

AU - Fujiwara, Koichi

AU - Kajiuchi, Toshio

PY - 2005/3/1

Y1 - 2005/3/1

N2 - Aqueous two-phase partitioning is one of the excellent bioseparation systems for proteins and other biomolecules in water-rich solutions containing polymers or salts. To improve the partition properties of model proteins, chicken egg white (CEW) proteins were chemically modified with polyethyleneoxide-maleic anhydride (PEOMA) copolymer. The modified proteins were partitioned in polyethyleneoxide-maleic acid/potassium phosphate aqueous two-phase systems. Firstly, the contribution of the chemical modification of proteins to the partition coefficient was investigated by using modified proteins with different degrees of modification (DM). The partition coefficients of modified proteins increased with increasing DM. The results suggested that the surface of the modified proteins had a greater hydrophobic property, similar to the PEOMA copolymer. Secondly, the effect of the pH on the partitioning of the modified proteins was studied. The partitioning of the modified proteins was found to be more dependent on pH in the alkaline systems. The increasing partition coefficient with pH at alkaline pH can be explained by the electrostatic repulsion between the phosphate anions and the maleic groups attached to the proteins. These results suggested that chemical modification of proteins with PEOMA could be a useful tool for separation and purification of crude protein mixtures.

AB - Aqueous two-phase partitioning is one of the excellent bioseparation systems for proteins and other biomolecules in water-rich solutions containing polymers or salts. To improve the partition properties of model proteins, chicken egg white (CEW) proteins were chemically modified with polyethyleneoxide-maleic anhydride (PEOMA) copolymer. The modified proteins were partitioned in polyethyleneoxide-maleic acid/potassium phosphate aqueous two-phase systems. Firstly, the contribution of the chemical modification of proteins to the partition coefficient was investigated by using modified proteins with different degrees of modification (DM). The partition coefficients of modified proteins increased with increasing DM. The results suggested that the surface of the modified proteins had a greater hydrophobic property, similar to the PEOMA copolymer. Secondly, the effect of the pH on the partitioning of the modified proteins was studied. The partitioning of the modified proteins was found to be more dependent on pH in the alkaline systems. The increasing partition coefficient with pH at alkaline pH can be explained by the electrostatic repulsion between the phosphate anions and the maleic groups attached to the proteins. These results suggested that chemical modification of proteins with PEOMA could be a useful tool for separation and purification of crude protein mixtures.

UR - http://www.scopus.com/inward/record.url?scp=14844307613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14844307613&partnerID=8YFLogxK

U2 - 10.1002/jctb.1210

DO - 10.1002/jctb.1210

M3 - Article

VL - 80

SP - 338

EP - 344

JO - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 3

ER -