Chemicals that modulate stem cell differentiation

Ki Chul Hwang, Ji Young Kim, Woochul Chang, Dae Sung Kim, Soyeon Lim, Sang Moon Kang, Byeong Wook Song, Hye Yeong Ha, Yong Joon Huh, In Geol Choi, Dong Youn Hwang, Heesang Song, Yangsoo Jang, Namsik Chung, Sung Hou Kim, Dong Wook Kim

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Important cellular processes such as cell fate are likely to be controlled by an elaborate orchestration of multiple signaling pathways, many of which are still not well understood or known. Because protein kinases, the members of a large family of proteins involved in modulating many known signaling pathways, are likely to play important roles in balancing multiple signals to modulate cell fate, we focused our initial search for chemical reagents that regulate stem cell fate among known inhibitors of protein kinases. We have screened 41 characterized inhibitors of six major protein kinase subfamilies to alter the orchestration of multiple signaling pathways involved in differentiation of stem cells. We found that some of them cause recognizable changes in the differentiation rates of two types of stem cells, rat mesenchymal stem cells (MSCs) and mouse embryonic stem cells (ESCs). Among many, we describe the two most effective derivatives of the same scaffold compound, isoquinolinesulfonamide, on the stem cell differentiation: rat MSCs to chondrocytes and mouse ESCs to dopaminergic neurons.

Original languageEnglish
Pages (from-to)7467-7471
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number21
DOIs
Publication statusPublished - 2008 May 27

Fingerprint

Cell Differentiation
Stem Cells
Mesenchymal Stromal Cells
Protein Kinases
Dopaminergic Neurons
Protein Kinase Inhibitors
Chondrocytes
Proteins
Mouse Embryonic Stem Cells

All Science Journal Classification (ASJC) codes

  • General

Cite this

Hwang, K. C., Kim, J. Y., Chang, W., Kim, D. S., Lim, S., Kang, S. M., ... Kim, D. W. (2008). Chemicals that modulate stem cell differentiation. Proceedings of the National Academy of Sciences of the United States of America, 105(21), 7467-7471. https://doi.org/10.1073/pnas.0802825105
Hwang, Ki Chul ; Kim, Ji Young ; Chang, Woochul ; Kim, Dae Sung ; Lim, Soyeon ; Kang, Sang Moon ; Song, Byeong Wook ; Ha, Hye Yeong ; Huh, Yong Joon ; Choi, In Geol ; Hwang, Dong Youn ; Song, Heesang ; Jang, Yangsoo ; Chung, Namsik ; Kim, Sung Hou ; Kim, Dong Wook. / Chemicals that modulate stem cell differentiation. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 21. pp. 7467-7471.
@article{ef47e4093b934ea9a045cfab4b439b04,
title = "Chemicals that modulate stem cell differentiation",
abstract = "Important cellular processes such as cell fate are likely to be controlled by an elaborate orchestration of multiple signaling pathways, many of which are still not well understood or known. Because protein kinases, the members of a large family of proteins involved in modulating many known signaling pathways, are likely to play important roles in balancing multiple signals to modulate cell fate, we focused our initial search for chemical reagents that regulate stem cell fate among known inhibitors of protein kinases. We have screened 41 characterized inhibitors of six major protein kinase subfamilies to alter the orchestration of multiple signaling pathways involved in differentiation of stem cells. We found that some of them cause recognizable changes in the differentiation rates of two types of stem cells, rat mesenchymal stem cells (MSCs) and mouse embryonic stem cells (ESCs). Among many, we describe the two most effective derivatives of the same scaffold compound, isoquinolinesulfonamide, on the stem cell differentiation: rat MSCs to chondrocytes and mouse ESCs to dopaminergic neurons.",
author = "Hwang, {Ki Chul} and Kim, {Ji Young} and Woochul Chang and Kim, {Dae Sung} and Soyeon Lim and Kang, {Sang Moon} and Song, {Byeong Wook} and Ha, {Hye Yeong} and Huh, {Yong Joon} and Choi, {In Geol} and Hwang, {Dong Youn} and Heesang Song and Yangsoo Jang and Namsik Chung and Kim, {Sung Hou} and Kim, {Dong Wook}",
year = "2008",
month = "5",
day = "27",
doi = "10.1073/pnas.0802825105",
language = "English",
volume = "105",
pages = "7467--7471",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "21",

}

Hwang, KC, Kim, JY, Chang, W, Kim, DS, Lim, S, Kang, SM, Song, BW, Ha, HY, Huh, YJ, Choi, IG, Hwang, DY, Song, H, Jang, Y, Chung, N, Kim, SH & Kim, DW 2008, 'Chemicals that modulate stem cell differentiation', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 21, pp. 7467-7471. https://doi.org/10.1073/pnas.0802825105

Chemicals that modulate stem cell differentiation. / Hwang, Ki Chul; Kim, Ji Young; Chang, Woochul; Kim, Dae Sung; Lim, Soyeon; Kang, Sang Moon; Song, Byeong Wook; Ha, Hye Yeong; Huh, Yong Joon; Choi, In Geol; Hwang, Dong Youn; Song, Heesang; Jang, Yangsoo; Chung, Namsik; Kim, Sung Hou; Kim, Dong Wook.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 21, 27.05.2008, p. 7467-7471.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemicals that modulate stem cell differentiation

AU - Hwang, Ki Chul

AU - Kim, Ji Young

AU - Chang, Woochul

AU - Kim, Dae Sung

AU - Lim, Soyeon

AU - Kang, Sang Moon

AU - Song, Byeong Wook

AU - Ha, Hye Yeong

AU - Huh, Yong Joon

AU - Choi, In Geol

AU - Hwang, Dong Youn

AU - Song, Heesang

AU - Jang, Yangsoo

AU - Chung, Namsik

AU - Kim, Sung Hou

AU - Kim, Dong Wook

PY - 2008/5/27

Y1 - 2008/5/27

N2 - Important cellular processes such as cell fate are likely to be controlled by an elaborate orchestration of multiple signaling pathways, many of which are still not well understood or known. Because protein kinases, the members of a large family of proteins involved in modulating many known signaling pathways, are likely to play important roles in balancing multiple signals to modulate cell fate, we focused our initial search for chemical reagents that regulate stem cell fate among known inhibitors of protein kinases. We have screened 41 characterized inhibitors of six major protein kinase subfamilies to alter the orchestration of multiple signaling pathways involved in differentiation of stem cells. We found that some of them cause recognizable changes in the differentiation rates of two types of stem cells, rat mesenchymal stem cells (MSCs) and mouse embryonic stem cells (ESCs). Among many, we describe the two most effective derivatives of the same scaffold compound, isoquinolinesulfonamide, on the stem cell differentiation: rat MSCs to chondrocytes and mouse ESCs to dopaminergic neurons.

AB - Important cellular processes such as cell fate are likely to be controlled by an elaborate orchestration of multiple signaling pathways, many of which are still not well understood or known. Because protein kinases, the members of a large family of proteins involved in modulating many known signaling pathways, are likely to play important roles in balancing multiple signals to modulate cell fate, we focused our initial search for chemical reagents that regulate stem cell fate among known inhibitors of protein kinases. We have screened 41 characterized inhibitors of six major protein kinase subfamilies to alter the orchestration of multiple signaling pathways involved in differentiation of stem cells. We found that some of them cause recognizable changes in the differentiation rates of two types of stem cells, rat mesenchymal stem cells (MSCs) and mouse embryonic stem cells (ESCs). Among many, we describe the two most effective derivatives of the same scaffold compound, isoquinolinesulfonamide, on the stem cell differentiation: rat MSCs to chondrocytes and mouse ESCs to dopaminergic neurons.

UR - http://www.scopus.com/inward/record.url?scp=44949240499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44949240499&partnerID=8YFLogxK

U2 - 10.1073/pnas.0802825105

DO - 10.1073/pnas.0802825105

M3 - Article

C2 - 18480249

AN - SCOPUS:44949240499

VL - 105

SP - 7467

EP - 7471

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 21

ER -