Chemicals that modulate stem cell differentiation

Ki Chul Hwang, Ji Young Kim, Woochul Chang, Dae Sung Kim, Soyeon Lim, Sang Moon Kang, Byeong Wook Song, Hye Yeong Ha, Yong Joon Huh, In Geol Choi, Dong Youn Hwang, Heesang Song, Yangsoo Jang, Namsik Chung, Sung Hou Kim, Dong Wook Kim

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37 Citations (Scopus)

Abstract

Important cellular processes such as cell fate are likely to be controlled by an elaborate orchestration of multiple signaling pathways, many of which are still not well understood or known. Because protein kinases, the members of a large family of proteins involved in modulating many known signaling pathways, are likely to play important roles in balancing multiple signals to modulate cell fate, we focused our initial search for chemical reagents that regulate stem cell fate among known inhibitors of protein kinases. We have screened 41 characterized inhibitors of six major protein kinase subfamilies to alter the orchestration of multiple signaling pathways involved in differentiation of stem cells. We found that some of them cause recognizable changes in the differentiation rates of two types of stem cells, rat mesenchymal stem cells (MSCs) and mouse embryonic stem cells (ESCs). Among many, we describe the two most effective derivatives of the same scaffold compound, isoquinolinesulfonamide, on the stem cell differentiation: rat MSCs to chondrocytes and mouse ESCs to dopaminergic neurons.

Original languageEnglish
Pages (from-to)7467-7471
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number21
DOIs
Publication statusPublished - 2008 May 27

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Hwang, K. C., Kim, J. Y., Chang, W., Kim, D. S., Lim, S., Kang, S. M., Song, B. W., Ha, H. Y., Huh, Y. J., Choi, I. G., Hwang, D. Y., Song, H., Jang, Y., Chung, N., Kim, S. H., & Kim, D. W. (2008). Chemicals that modulate stem cell differentiation. Proceedings of the National Academy of Sciences of the United States of America, 105(21), 7467-7471. https://doi.org/10.1073/pnas.0802825105