A lateral flow immunoassay (LF-immunoassay) with an enhanced sensitivity and thermostability was developed by using Pt nanoparticles with a peroxidase activity. The Pt nanoparticles were synthesized by citrate reduction method, and the peroxidase activity of Pt nanoparticles was optimized by adjusting reaction conditions. The peroxidase activity was estimated by using Michaelis-Menten kinetics model with TMB as a chromogenic substrate. The kinetics parameters of KM and Vmax were calculated and compared with horseradish peroxidase (HRP). The thermal stability of the Pt nanoparticles was compared with horseradish peroxidase (HRP) according to the storage temperature and long-term storage period. The feasibility of lateral flow immunoassay with a chemiluminescent signal band was demonstrated by the detection of human chorionic gonadotropin (hCG) as a model analyte, and the sensitivity was determined to be improved by as much as 1000-fold compared to the conventional rapid test based on colored gold-colloids.
Bibliographical noteFunding Information:
This research was supported by the National Research Foundation of Korea ( 2013-056267 , 2013-056331 and 2013R1A1A2065723 ). This work was also supported by the Creative Fusion Research Program through the Creative Allied Project funded by Korea Research Council of Fundamental Science and Technology ( CAP-12-1 ).
© 2014 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Environmental Chemistry