Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

Jung Seok Lee; Jong Bin Lee; Jae Kook Cha; Eun Young Choi; So Yon Park; Kyoo Sung Cho; Chang Sung Kim

doi:10.1111/jcpe.12710

Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

Jung Seok Lee, Jong Bin Lee, Jae Kook Cha, Eun Young Choi, So Yon Park, Kyoo Sung Cho, Chang Sung Kim

Department of Periodontics

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.

Original language	English
Pages (from-to)	530-539
Number of pages	10
Journal	Journal of Clinical Periodontology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1111/jcpe.12710
Publication status	Published - 2017 May

Bibliographical note

Funding Information:
Conflict of interest and source of funding statement Prof. Kim has a patent pending on the method of isolation stem cell using transwell and chemokine, and the other authors have stated explicitly that there are no conflicts of interest in connection with this article. This research was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP; No. 2015R1A2A1A15053961) and the Ministry of Science, ICT & Future Planning (No. 2012M3A9B2052521).

Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

All Science Journal Classification (ASJC) codes

Periodontics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/jcpe.12710

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title = "Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration",

abstract = "Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.",

author = "Lee, {Jung Seok} and Lee, {Jong Bin} and Cha, {Jae Kook} and Choi, {Eun Young} and Park, {So Yon} and Cho, {Kyoo Sung} and Kim, {Chang Sung}",

note = "Funding Information: Conflict of interest and source of funding statement Prof. Kim has a patent pending on the method of isolation stem cell using transwell and chemokine, and the other authors have stated explicitly that there are no conflicts of interest in connection with this article. This research was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP; No. 2015R1A2A1A15053961) and the Ministry of Science, ICT & Future Planning (No. 2012M3A9B2052521). Publisher Copyright: {\textcopyright} 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

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month = may,

doi = "10.1111/jcpe.12710",

language = "English",

volume = "44",

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T1 - Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

AU - Lee, Jung Seok

AU - Lee, Jong Bin

AU - Cha, Jae Kook

AU - Choi, Eun Young

AU - Park, So Yon

AU - Cho, Kyoo Sung

AU - Kim, Chang Sung

N1 - Funding Information: Conflict of interest and source of funding statement Prof. Kim has a patent pending on the method of isolation stem cell using transwell and chemokine, and the other authors have stated explicitly that there are no conflicts of interest in connection with this article. This research was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP; No. 2015R1A2A1A15053961) and the Ministry of Science, ICT & Future Planning (No. 2012M3A9B2052521). Publisher Copyright: © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

PY - 2017/5

Y1 - 2017/5

N2 - Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.

AB - Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.

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VL - 44

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JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

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Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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