Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

Jung Seok Lee, Jong Bin Lee, Jae Kook Cha, Eun Young Choi, So Yon Park, Kyoo Sung Cho, Chang Sung Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.

Original languageEnglish
Pages (from-to)530-539
Number of pages10
JournalJournal of Clinical Periodontology
Volume44
Issue number5
DOIs
Publication statusPublished - 2017 May

Bibliographical note

Funding Information:
Conflict of interest and source of funding statement Prof. Kim has a patent pending on the method of isolation stem cell using transwell and chemokine, and the other authors have stated explicitly that there are no conflicts of interest in connection with this article. This research was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP; No. 2015R1A2A1A15053961) and the Ministry of Science, ICT & Future Planning (No. 2012M3A9B2052521).

Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

All Science Journal Classification (ASJC) codes

  • Periodontics

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