Abstract
Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.
| Original language | English |
|---|---|
| Pages (from-to) | 530-539 |
| Number of pages | 10 |
| Journal | Journal of Clinical Periodontology |
| Volume | 44 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2017 May |
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All Science Journal Classification (ASJC) codes
- Periodontics
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Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration. / Lee, Jung Seok; Lee, Jong Bin; Cha, Jae Kook; Choi, Eun Young; Park, So Yon; Cho, Kyoo Sung; Kim, Chang Sung.
In: Journal of Clinical Periodontology, Vol. 44, No. 5, 05.2017, p. 530-539.Research output: Contribution to journal › Article
TY - JOUR
T1 - Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration
AU - Lee, Jung Seok
AU - Lee, Jong Bin
AU - Cha, Jae Kook
AU - Choi, Eun Young
AU - Park, So Yon
AU - Cho, Kyoo Sung
AU - Kim, Chang Sung
PY - 2017/5
Y1 - 2017/5
N2 - Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.
AB - Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and Methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of “appearing,” “increasing,” and “decreasing/disappearing” from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.
UR - http://www.scopus.com/inward/record.url?scp=85017405591&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017405591&partnerID=8YFLogxK
U2 - 10.1111/jcpe.12710
DO - 10.1111/jcpe.12710
M3 - Article
C2 - 28207939
AN - SCOPUS:85017405591
VL - 44
SP - 530
EP - 539
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
SN - 0303-6979
IS - 5
ER -