Chemokine receptor CCR3 expression in malignant cutaneous tumors

Yoon Jin Lee, Dae Hyun Kim, Sang Han Lee, Hae Seon Nam, MiRyung Roh, Moon Kyun Cho

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Chemokines and their receptors are important players in tumorigenesis by facilitating tumor proliferation and metastasis. Little is known about the possible function of chemokine receptors in relation to the development and progression of malignant cutaneous tumors. Objective: The aim of this study was to determine the chemokine receptor CCR3 expression pattern and the protein expression level in selected malignant cutaneous tumors. Methods: Four types of cell lines (G361, A431, SK-MEL-2, SK-MEL-24) were analyzed, using Western blotting, for the expression of CCR3 protein. Immunohistochemical staining for CCR3 was done on 36 skin cancer tissue samples that included 16 squamous cell carcinomas (SCCs), 16 basal cell carcinomas (BCCs), 16 malignant melanomas (MMs) and 6 normal tissue samples. Results: Western blot analysis showed that CCR3 protein was more expressed in the MM cell lines (G361, SK-MEL-2, SK-MEL-24) than that in the SCC cell line (A431), and the immunohistochemical analysis showed that CCR3 protein was overexpressed in MM and SCC, it was mildly expressed in BCC and it was hardly expressed in normal tissue. Conclusion: This study demonstrated via immunochemistry that CCR3 was more expressed in MM, followed by SCC and BCC. The existence of CCR3 protein may enhance the tumorigenic potential of malignant cutaneous tumors.

Original languageEnglish
Pages (from-to)412-417
Number of pages6
JournalAnnals of Dermatology
Volume22
Issue number4
DOIs
Publication statusPublished - 2010 Nov 1

Fingerprint

Chemokine Receptors
Squamous Cell Carcinoma
Melanoma
Basal Cell Carcinoma
Skin
Neoplasms
Proteins
Cell Line
Western Blotting
Immunochemistry
Skin Neoplasms
Carcinogenesis
Staining and Labeling
Neoplasm Metastasis

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Lee, Yoon Jin ; Kim, Dae Hyun ; Lee, Sang Han ; Nam, Hae Seon ; Roh, MiRyung ; Cho, Moon Kyun. / Chemokine receptor CCR3 expression in malignant cutaneous tumors. In: Annals of Dermatology. 2010 ; Vol. 22, No. 4. pp. 412-417.
@article{245a2ac707464b89be1dc04c8214f1df,
title = "Chemokine receptor CCR3 expression in malignant cutaneous tumors",
abstract = "Background: Chemokines and their receptors are important players in tumorigenesis by facilitating tumor proliferation and metastasis. Little is known about the possible function of chemokine receptors in relation to the development and progression of malignant cutaneous tumors. Objective: The aim of this study was to determine the chemokine receptor CCR3 expression pattern and the protein expression level in selected malignant cutaneous tumors. Methods: Four types of cell lines (G361, A431, SK-MEL-2, SK-MEL-24) were analyzed, using Western blotting, for the expression of CCR3 protein. Immunohistochemical staining for CCR3 was done on 36 skin cancer tissue samples that included 16 squamous cell carcinomas (SCCs), 16 basal cell carcinomas (BCCs), 16 malignant melanomas (MMs) and 6 normal tissue samples. Results: Western blot analysis showed that CCR3 protein was more expressed in the MM cell lines (G361, SK-MEL-2, SK-MEL-24) than that in the SCC cell line (A431), and the immunohistochemical analysis showed that CCR3 protein was overexpressed in MM and SCC, it was mildly expressed in BCC and it was hardly expressed in normal tissue. Conclusion: This study demonstrated via immunochemistry that CCR3 was more expressed in MM, followed by SCC and BCC. The existence of CCR3 protein may enhance the tumorigenic potential of malignant cutaneous tumors.",
author = "Lee, {Yoon Jin} and Kim, {Dae Hyun} and Lee, {Sang Han} and Nam, {Hae Seon} and MiRyung Roh and Cho, {Moon Kyun}",
year = "2010",
month = "11",
day = "1",
doi = "10.5021/ad.2010.22.4.412",
language = "English",
volume = "22",
pages = "412--417",
journal = "Annals of Dermatology",
issn = "1013-9087",
publisher = "Korean Dermatological Association",
number = "4",

}

Chemokine receptor CCR3 expression in malignant cutaneous tumors. / Lee, Yoon Jin; Kim, Dae Hyun; Lee, Sang Han; Nam, Hae Seon; Roh, MiRyung; Cho, Moon Kyun.

In: Annals of Dermatology, Vol. 22, No. 4, 01.11.2010, p. 412-417.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemokine receptor CCR3 expression in malignant cutaneous tumors

AU - Lee, Yoon Jin

AU - Kim, Dae Hyun

AU - Lee, Sang Han

AU - Nam, Hae Seon

AU - Roh, MiRyung

AU - Cho, Moon Kyun

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Background: Chemokines and their receptors are important players in tumorigenesis by facilitating tumor proliferation and metastasis. Little is known about the possible function of chemokine receptors in relation to the development and progression of malignant cutaneous tumors. Objective: The aim of this study was to determine the chemokine receptor CCR3 expression pattern and the protein expression level in selected malignant cutaneous tumors. Methods: Four types of cell lines (G361, A431, SK-MEL-2, SK-MEL-24) were analyzed, using Western blotting, for the expression of CCR3 protein. Immunohistochemical staining for CCR3 was done on 36 skin cancer tissue samples that included 16 squamous cell carcinomas (SCCs), 16 basal cell carcinomas (BCCs), 16 malignant melanomas (MMs) and 6 normal tissue samples. Results: Western blot analysis showed that CCR3 protein was more expressed in the MM cell lines (G361, SK-MEL-2, SK-MEL-24) than that in the SCC cell line (A431), and the immunohistochemical analysis showed that CCR3 protein was overexpressed in MM and SCC, it was mildly expressed in BCC and it was hardly expressed in normal tissue. Conclusion: This study demonstrated via immunochemistry that CCR3 was more expressed in MM, followed by SCC and BCC. The existence of CCR3 protein may enhance the tumorigenic potential of malignant cutaneous tumors.

AB - Background: Chemokines and their receptors are important players in tumorigenesis by facilitating tumor proliferation and metastasis. Little is known about the possible function of chemokine receptors in relation to the development and progression of malignant cutaneous tumors. Objective: The aim of this study was to determine the chemokine receptor CCR3 expression pattern and the protein expression level in selected malignant cutaneous tumors. Methods: Four types of cell lines (G361, A431, SK-MEL-2, SK-MEL-24) were analyzed, using Western blotting, for the expression of CCR3 protein. Immunohistochemical staining for CCR3 was done on 36 skin cancer tissue samples that included 16 squamous cell carcinomas (SCCs), 16 basal cell carcinomas (BCCs), 16 malignant melanomas (MMs) and 6 normal tissue samples. Results: Western blot analysis showed that CCR3 protein was more expressed in the MM cell lines (G361, SK-MEL-2, SK-MEL-24) than that in the SCC cell line (A431), and the immunohistochemical analysis showed that CCR3 protein was overexpressed in MM and SCC, it was mildly expressed in BCC and it was hardly expressed in normal tissue. Conclusion: This study demonstrated via immunochemistry that CCR3 was more expressed in MM, followed by SCC and BCC. The existence of CCR3 protein may enhance the tumorigenic potential of malignant cutaneous tumors.

UR - http://www.scopus.com/inward/record.url?scp=79955607725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955607725&partnerID=8YFLogxK

U2 - 10.5021/ad.2010.22.4.412

DO - 10.5021/ad.2010.22.4.412

M3 - Article

VL - 22

SP - 412

EP - 417

JO - Annals of Dermatology

JF - Annals of Dermatology

SN - 1013-9087

IS - 4

ER -